Extraction of genomic DNA from wild boar (Sus scrofa) muscle tissue using hydrophobic magnetic deep eutectic solvents

Extraction of high‐quality genomic DNA from wild boar tissue is of great significance for the study of its genetic information, population quantity, and distribution. In this work, a hydrophobic magnetic deep eutectic solvent (HMDES) was synthesized. Based on the HMDES, a HMDES‐based vortexed extrac...

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Bibliographic Details
Published in:Journal of the Chinese Chemical Society (Taipei) 2024-08, Vol.71 (8), p.756-762
Main Authors: He, Xingchen, Ma, Yihan, Wang, Hongyu, Ma, Yani, Li, Qian, Tang, Xin, Mo, Xiaoyang, Ding, Xueqin
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
genomic DNA extraction from wild boar (Sus scrofa)
green extraction
hydrophobic magnetic deep eutectic solvent
Hydrophobicity
magnetic solvent
Muscles
Solvents
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Summary:Extraction of high‐quality genomic DNA from wild boar tissue is of great significance for the study of its genetic information, population quantity, and distribution. In this work, a hydrophobic magnetic deep eutectic solvent (HMDES) was synthesized. Based on the HMDES, a HMDES‐based vortexed extraction method was developed for extracting genomic DNA from wild boar muscle tissue. Compared to the traditional high salt‐extraction method, this approach is faster and embraces higher DNA extraction efficiency. Different pretreatment methods before extraction were evaluated. Single‐factor experiments were used to optimize the extraction condition, for example, extraction time, temperature, and HMDES volume. After extraction, the DNA can be quickly and easily recovered from the HMDES phase, and the HMDES can be reused. This work provides a simple and environmental‐friendly extraction method for DNA extraction from wild boar tissue. In this study, a hydrophobic magnetic deep eutectic solvent (HMDES) based vortexed extraction was developed for extraction of genomic DNA from wild boar (Sus scrofa) muscle tissue. Compared to traditional high salt‐extraction, the HMDES‐based vortexed extraction method is simple, convenient, and has higher extraction efficiency. This work provides a green extraction solvent and an environmental‐friendly extraction method for nucleic acids extraction from animal tissue.
ISSN:0009-4536
2192-6549
DOI:10.1002/jccs.202400096