Protein profiles of follicle fluid of different sizes in cows and buffaloes

In vitro embryo production systems in buffaloes have great productive perspectives and opportunities for improvement. Among these, comparative studies with species with more significant advances in reproductive biotechnology have been developed. Accordingly, this work aimed to identify the differenc...

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Bibliographic Details
Published in:Revista Facultad Nacional de Agronomía, Medellín Medellín, 2024-01, Vol.77 (1), p.10651
Main Authors: Jesús Alfredo Berdugo Gutiérrez, Angela Patricia Brijaldo Villamizar, Albeiro López Herrera, Verónica González Cadavid, Fabian Leonardo Rueda Alfonso
Format: Article
Language:English
Subjects:
Abattoirs
Albumin
Albumins
Biotechnology
Bubalus bubalis
Buffalo
Cattle
Comparative studies
Electrophoresis
Follicles
Follicular fluid
Gels
Immunoglobulins
Mann-Whitney U test
Molecular weight
Ovaries
Polyacrylamide
Proteins
Sodium dodecyl sulfate
Sodium lauryl sulfate
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Summary:In vitro embryo production systems in buffaloes have great productive perspectives and opportunities for improvement. Among these, comparative studies with species with more significant advances in reproductive biotechnology have been developed. Accordingly, this work aimed to identify the differences in the electrophoretic profiles of proteins in the follicular fluid (FF) of cows and buffaloes and their possible association with follicle size. FF was obtained at the central abattoir in Medellín (Antioquia), Colombia, from small (7 mm) follicles from the ovaries of 25 cows (Bos indicus) and 20 buffaloes (Bubalus bubalis). The total protein content of the FF was quantified and subsequently depleted of albumin and immunoglobulins. Samples were subjected to denaturing electrophoresis in sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) to determine the electrophoretic profiles using a photodocumenter. The values obtained for the relative amount of each band were compared between species and follicle sizes using the Mann-Whitney test. The results showed no significant differences in total protein concentration between the different follicle sizes and species. Further, 72.6% of the FF proteins are immunoglobulins and albumin. The profiles of small follicles (
ISSN:0304-2847
2248-7026
DOI:10.15446/rfnam.v77n1.108022