Liquid pineapple waste as substrate for the production of bacterial nanocellulose (BNC) by local isolated microbe Kc-D-4

• Main Authors: Mulyono, Nurbaiti, Rilyanti, Mita, Herasari, Dian
• Format: Conference Proceeding
• Language: English
• Subjects: Bacteria; Biocompatibility; Carbon; Chemical properties; Glucose; Liquid wastes; Mechanical properties; Pellicle; Pineapples; Production costs; Scanning electron microscopy; Structural analysis; Substrates
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0210870

Over the closing decade, bacterial nanocellulose (BNC) has earned growing international attention due to its great physical and chemical properties, such as green processing, low production cost, multiplied mechanical properties, hydrophilicity, outstanding biocompatibility, and biodegradability. BNC production generally uses commercial glucose as a substrate in a liquid Hestrin-Schramm medium. Using natural carbon sources such as glucose-rich pineapple liquid waste as a source of alternate carbon in place of glucose is expected to minimize production costs. This study aimed to obtain isolates of BNC-producing bacteria and find the potential of pineapple liquid waste as a carbon source in BNC production. Here, we’re reporting the BNC production by substituting glucose in liquid Hestrin-Schramm medium using pineapple liquid waste with variations in concentrations in Brix of 2, 4, 6, and 8. BNC production was carried out at a static state with an incubation time of 7, 14, and 21 days. The parameters measured include the weight of the BNC pellicle produced and the water hold capacity (WHC) and characterization using scanning electron microscopy (SEM). The results showed that the Kc-D-4 isolate was selected as the best isolate and produced a halo index of 2.6 in GEY medium. Optimum fermentation conditions for the production of BNC by Kc-D-4 isolates were achieved at a pineapple liquid waste as a carbon source with a Brix of 8 at an incubation time of 14 days in a static state. BNC pellicles weighing 10.2 grams with a WHC of 97.3% were produced in these conditions. SEM characterization showed that the pellicle structure has a nano-size with a diameter of 39-92 nm. Thus, pineapple liquid waste has the potential to be used as a substrate to produce BNC.