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Studies On Synergistic Effects of Lambda-Cyhalothrin and Methylcobalamin (Vitamin B12) On Development Of Chick Embryo

Purpose: Chicken embryos serve as a prominent model in embryonic development research. The present study evaluates the developmental impact of Lambda-Cyhalothrin and Methylcobalamin (Vitamin B12) on Gallus domisticus chick embryos. Methods: 250 fertilized eggs of Gallus domesticus were distributed i...

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Published in:Fluoride 2024-07, Vol.57 (8), p.1-10
Main Authors: Khalid, Rameen, Ahmad, Syeda Nadia, Inayat, Iram, Suleman, Sadia, Kanwal, Muhammad Ali, Batool, Aima Iram, Kamran, Muhammad Atif, Younis, Asma, Khalid, Moattar, Ahmad, Khawaja Raees
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Language:English
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Summary:Purpose: Chicken embryos serve as a prominent model in embryonic development research. The present study evaluates the developmental impact of Lambda-Cyhalothrin and Methylcobalamin (Vitamin B12) on Gallus domisticus chick embryos. Methods: 250 fertilized eggs of Gallus domesticus were distributed into five groups: i) Control group (CG): incubated without any treatment ii) Vehicle Control group (VC): injected with 0.1mL of a 5% DMSO solution in water iii) Lambda-Cyhalothrin group (LCH): injected with 0.1mL of 0.01mg/kg LCH solution in 5% DMSO iv) Vitamin B12 group (B12): injected with 0.1mL of 0.1mg/kg vitamin B12 solution in 5% DMSO v) Lambda-Cyhalothrin group + Vitamin B12 (LCH+B12): injected with 0.1mL of 0.01 mg/kg LCH 0.1mg/kg vitamin B12 solution. Treatment was given on zero day and embryos were recovered on 14th day after incubation to check embryonic development. Results: Morphological observations indicated that both the CG and VC groups displayed normal development. In contrast, the LCH and LCH+B12 group exhibited adverse effects, which included embryonic cataracts, neck deformities, muscular dystrophy, and a higher rate of embryo mortality when compared to the CG and VC groups. Moreover, LCH+B12 group also showed severe synergistic embryonic toxicity, characterized by halted development, foul odor, sulfur contents, blackish discoloration and teratogenic signs including embryonic cataract, evisceration, halted neck, hemorrhage spot and reduced limb size. Conclusion: Type-II fluoridated pyrethroid LCH is highly toxic to chick development. Study revealed that Vitamin B12 alone does not interfere with normal development however its co-treatment with LCH can aggravate toxic potentials of LCH for embryonic development in the chick.
ISSN:0015-4725
2253-4083