Spectroscopic Studies on Plant Extract Mediated ZnO Nanoparticles as a Potential Cytotoxic Agent

Plants play an important role in nanoparticle preparation because they are easily accessible, environmentally friendly, and inexpensive. In this study, we used an ethanolic extract of Mangifera indica seed as a reducing and stabilising agent to create zinc oxide (ZnO) nanoparticles (NPs). The ZnO NP...

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Bibliographic Details
Published in:Journal of applied spectroscopy 2024-09, Vol.91 (4), p.912-920
Main Authors: Delma, B. T., Princela, M. Antilin, Subbareddy, Y., Malbi, M. Anitha, Roselet, S. Lizy, Treasa, M. Shirly, Rao, M. C.
Format: Article
Language:English
Subjects:
Absorption
Analytical Chemistry
Atomic/Molecular Structure and Spectra
Biological effects
Cytotoxicity
Fourier transforms
Infrared spectroscopy
Metal oxides
Metallurgical constituents
Nanoparticles
Physics
Physics and Astronomy
Plant layout
Scanning electron microscopy
Stabilizers (agents)
Transmission electron microscopy
X-ray diffraction
Zinc oxide
Zinc oxides
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Summary:Plants play an important role in nanoparticle preparation because they are easily accessible, environmentally friendly, and inexpensive. In this study, we used an ethanolic extract of Mangifera indica seed as a reducing and stabilising agent to create zinc oxide (ZnO) nanoparticles (NPs). The ZnO NPs were examined using characterization techniques such as UV-Vis, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The interaction of phytochemical constituents from plant extracts providing the biological reduction of zinc metal ions to ZnO had been identified by the UV-visible absorption studies. According to the FT-IR results, metal oxides exhibited interatomic vibration-driven absorption in the fingerprint area below 1000 cm –1 . Particles appeared to be crystalline and also a rice-grain shape of ZnO NPs was confirmed by XRD, SEM, and TEM, respectively. In addition, the cytotoxic effect of ZnO NPs was checked using the SKMEL-28 cell line, showing an IC50 value of 32.686 μg/mL in the SKMEL-28 cell line, and 49.011 μg/mL in the typical L6 cell line. Furthermore, the synthesized NPs were subjected to (AO/EB) double staining approach to examine the apoptotic activity. The acridine orange/ethidium bromide method made strong evidence for demonstrating chromatin condensation and membrane blebbing.
ISSN:0021-9037
1573-8647
DOI:10.1007/s10812-024-01799-5