Hepatoprotective Effects of Tamarix dioica Leaf Extracts on Paracetamol-Induced Hepatotoxicity in Mice

• Published in: Pakistan journal of zoology 2024-10, Vol.56 (5), p.2195
• Main Authors: Al-Matrafi, Tahani Ahmad, Mohammedsaleh, Zuhair M, Moawadh, Mamdoh S, Aggad, Waheeb S, Almuhimed, Rawabi Mohamed, Alhuwaymil, Zamzam, Albalawi, Aishah E, Alsharif, Ifat, Almohaimeed, Hailah M, Alaryani, Fatima S, Soliman, Mona H
• Format: Article
• Language: English
• Subjects: Acetaminophen; Albinism; Analgesics; Antioxidants; Bilirubin; Biomarkers; Complications and side effects; Cyclooxygenase-2; Diuretics; Drug dosages; Drugs; Flavonoids; Free radicals; Health aspects; Hepatitis; Hepatotoxicity; Herbal medicine; Indicators; Leaves; Liver; Liver cirrhosis; Liver diseases; Materia medica, Vegetable; Paracetamol; Pharmacology; Phenols; Physiology; Phytochemicals; Plant extracts; Prevention; Serum; Silymarin; Tamarisks; Tamarix dioica; Toxicity; Tumor necrosis factor-α
• ISSN: 0030-9923; 0030-9923
• DOI: 10.17582/journal.pjz/20230924082821

ABSTRACT This study aimed to evaluate the hepatoprotective effects of aqueous, methanolic, and ethanolic extracts of Tamarix dioica leaves against paracetamol-induced toxicity. In this study, 36 albino mice were randomly grouped into six groups, each consisting of six mice: Group I (normal control), Group II (paracetamol-toxified), Group III (positive control with Silymarin @ 200 mg/Kg), Group IV (aqueous T. dioica extract @ 400 mg/Kg), Group V (methanolic T. dioica extract @ 400 mg/Kg), and Group VI (ethanolic T. dioica extract @ 400 mg/Kg). Hepatoprotective potential was assessed through liver function indicators (ALT, AST, ALP, total bilirubin, and total protein in blood serums), hepatic antioxidants (SOD, CAT, GSH, and GPx in liver homogenate), and inflammatory markers (IL-6, TNF-α, COX2), along with other liver biomarkers. Histopathological alterations in the liver were evaluated using Hematoxylin and Eosin staining. The leaf extracts effectively restored liver function indicators and hepatic antioxidants to normal levels, demonstrating a significant improvement compared to the elevated levels observed in the paracetamol control group (P < 0.001). Furthermore, a reversal of hepatoarchitecture was recorded. The study highlights the pronounced hepatoprotective effects of T. dioica leaf extracts against paracetamol-induced toxicity in albino mice. The extracts not only successfully normalized liver function indicators and hepatic antioxidants but also exhibited a significant reversal of hepatoarchitecture. These findings suggest the potential therapeutic value of T. dioica in mitigating liver disorders, emphasizing its promising role as a natural hepatoprotective agent.