Phytochemical profiling and Immunomodulatory activity of belincong (Marsdenia brunoniana) fruit extract against Staphylococcus aureus infection in mice

• Published in: Research journal of pharmacy and technology 2024-08, Vol.17 (8), p.4022-4028
• Main Authors: Indah Savira, Nadyatul Ilma, Irsyad Maulana, Alfan, Hasanah, Mahfudhotul, Ihsani Zahra, Salma, Waluyo, Joko, Fikri, Kamalia, Wahyuni, Dwi, Syifa Fiarani, Hajar
• Format: Article
• Language: English
• Subjects: Antibiotics; Antibodies; Bioassays; Biodiversity; Dentistry; Drug resistance; Ethanol; Immune system; Immunoglobulins; Immunomodulators; Infectious diseases; Laboratories; Leukocytes; Phytochemicals; Spleen; Staphylococcus infections
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.52711/0974-360X.2024.00624

Marsdenia brunoniana, called belincong, is a wild plant growing in East Java, Indonesia. This study aimed to determine the phytochemical compounds and immunomodulatory activity of M. brunoniana fruit extract. Phytochemical screening of M. brunoniana fruit extract used GC-MS analysis. The immunomodulatory activity of M. brunoniana fruit extract was determined using the number of leukocytes, the weight of the spleen, phagocytic activity, and antibody level. There were 25 mice divided into five groups. Normal control (KN) was the group without any treatment, but negative control (K-) was the group with twice Staphylococcus aureus infection. The M. brunoniana fruit extract treatment groups were differentiated based on dose (P1= 50 mg/kg BW; P2= 100mg/kg BW; P3= 150mg/kg BW). M. brunoniana fruit extract was administered orally for two weeks, and Staphylococcus aureus was injected intraperitoneally after extract administration twice. The GC-MS analysis showed that M. brunoniana fruit extract has 28 compounds. The most compounds in the fruit extract were 1,2,3-Propanetriol (CAS) Glycerol, 1,4-Benzenediol (CAS) Hydroquinone, and Piperidine, 1-methyl- (CAS) N-Methylpiperidine. M. brunoniana fruit extract showed immunomodulatory activity by enhancing phagocytic activity and the weight of the spleen in the P2 group. In addition, the number of leukocytes returned to normal in the P1 group. Not only modulating the innate system, M. brunoniana fruit extract also significantly increased the level of antibodies in mice serum in the P3 group. Based on these results, phytochemical compounds of M. brunoniana fruit extract have immunomodulatory activity against Staphylococcus aureus infection.