Phytochemical diversity of Alpinia mutica Roxb. leaves extracts and their bioactivity correlations elucidated by nmr‐based metabolomics

• Published in: International journal of food science & technology 2024-10, Vol.59 (10), p.7907-7917
• Main Authors: Noorhadi, Najihah Hassan, Sofian‐Seng, Noor‐Soffalina, Teh, Arnida Hani, Afzan, Adlin, Misnan, Norazlan Mohmad, Mediani, Ahmed, Rahman, Hafeedza Abdul
• Format: Article
• Language: English
• Subjects: Alpinia mutica; antiobesity; antioxidant; Ascorbic acid; Biological activity; Catechin; Correlation; Diabetes mellitus; Ethanol; Ferric ions; Flavonoids; Free radicals; Glucosidase; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolites; Metabolomics; NMR; Nuclear magnetic resonance; pancreatic lipase inhibition; Phenolic compounds; Phenols; Phenylalanine; Phytochemicals; Scavenging; α‐glucosidase inhibition
• ISSN: 0950-5423; 1365-2621
• DOI: 10.1111/ijfs.17128

Summary Alpinia mutica Roxb. possesses diverse phytochemicals with potential as treatment modalities in managing diabetes and obesity. Employing a nuclear magnetic resonance (NMR)‐based metabolomics, we conducted discriminative analysis to investigate the metabolite profiles across different extraction solvents (ethanol:water −100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) and correlated these profiles with bioactivities. Metabolites were identified using NMR supplemented with liquid chromatography tandem mass spectrometry (LC–MS/MS) analysis. The 40:60 extract showed high phenolic content, strong ferric ion reduction capability and low IC50 value in scavenging free radicals, pancreatic lipase and α‐glucosidase inhibitory assays. Partial Least Square (PLS) biplot indicated that isorhamnetin, catechin, zerumin B, curcumanggoside, curcuzederone, demethoxycurcumin, ascorbic acid, apigenin, and phenylalanine in the 40:60 extract exhibited good correlations with the measured bioactivities. Additionally, LC–MS/MS tentatively identified proanthocyanidins and multiple flavonoid glucuronides in this extract. This study provides preliminary insight into its chemistry and highlight the potential of A. mutica extract as natural antioxidant and antiobesity agent. This study employed 1H NMR‐based metabolomics to assess the metabolites in Alpinia mutica Roxb. extracted using different solvents. The 40:60 extract exhibited high phenolic content, antioxidant activity, lipase, and α‐glucosidase inhibition. Isorhamnetin, catechin, zerumin B, curcumanggoside, curcuzederone, demethoxycurcumin, ascorbic acid, apigenin and phenylalanine in the 40:60 extract exhibited good correlations with the measured bioactivities.