Organelle-resolved imaging of formaldehyde reveals its spatiotemporal dynamics

Understanding the spatiotemporal dynamics of formaldehyde (FA) is crucial for elucidating its pathophysiology. In this study, we designed a series of organelle-resolved probes to investigate FA dynamics. By incorporating various organelle anchors into a coumarin hydrazonate, we developed a series of...

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Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2024-10, Vol.12 (38), p.9592-9599
Main Authors: Zhou, Lei, Pan, Yuan, Li, Xiaozhuan, Fan, Tingmin, Liang, Xingguang, Li, Xin
Format: Article
Language:English
Subjects:
Coumarin
Coumarins - chemistry
Coumarins - pharmacology
Dynamic response
Dynamics
Fluorescent Dyes - chemistry
Formaldehyde
Formaldehyde - chemistry
HeLa Cells
Humans
Molecular Structure
Optical Imaging
Organelles
Organelles - chemistry
Organelles - metabolism
Oxidative stress
Oxidative Stress - drug effects
Pathophysiology
Probes
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Summary:Understanding the spatiotemporal dynamics of formaldehyde (FA) is crucial for elucidating its pathophysiology. In this study, we designed a series of organelle-resolved probes to investigate FA dynamics. By incorporating various organelle anchors into a coumarin hydrazonate, we developed a series of probes with excellent organelle selectivity and FA specificity, enabling rapid and precise sensing of FA in an organelle-resolved way. Leveraging these probes, we captured the spatiotemporal dynamics of native FA in response to exogenous FA or oxidative stress challenges. In particular, we unveiled the distinct responses of various organelles to identical cellular stressors. Moreover, we observed the dynamic response within individual organelles when cells were exposed to stressors for varying durations. We envision these probes will serve as versatile tools for probing FA pathophysiology. Organelle-resolved imaging of formaldehyde reveals the different responses of various organelles to the same cell stressor.
ISSN:2050-750X
2050-7518
2050-7518
DOI:10.1039/d4tb01317d