Synthesis, Structural Characteristics, and Evaluation of the Antigen-Presenting Properties of Turpentine Oil Lyosol

• Published in: BioNanoScience 2024, Vol.14 (3), p.3254-3264
• Main Authors: Shibaeva, A. V., Bokareva, O. P., Tynyo, Y. Y., Trubnikova, E. V., Belyakova, A. V., Epova, E. Y., Sherbakova, E. S., Shabunina, M. A., Kuzmin, V. A.
• Format: Article
• Language: English
• Subjects: Biological and Medical Physics; Biomaterials; Biophysics; Circuits and Systems; Confocal microscopy; Dry matter; Engineering; Fluorescein; Monocytes; Nanofiltration; Nanoparticles; Nanotechnology; Oils & fats; Particle size; Phagocytosis; Synthesis; Turpentine
• ISSN: 2191-1630; 2191-1649
• DOI: 10.1007/s12668-024-01390-4

A method for the synthesis of a nanostructured turpentine oil lyosol by bubbling an emulsion of turpentine oil in water with air in a mass ratio of 1:200 to 1:2200 by the separation of nanoparticles using nanofiltration has been proposed. Changing the mass ratio of turpentine oil to the aqueous phase during the synthesis was shown to influence the size of the resulting nanoparticles. An increase in the Z-average size of nanoparticles from 50 to 320 nm along with a gradual decreasing turpentine content from 1:200 to 1:1000 was observed. A further decrease in the turpentine load from 1:1000 to 1:2200 led to decreasing the Z-average particle size up to 160 nm. The fact of the formation of stable non-covalent complexes of the lyosol particles with fluorescein has been established. The fluorescein-stained lyosol particles were used to study the efficiency of their phagocytosis by chicken blood monocytes using confocal microscopy. The maximum efficiency of the lyosol phagocytosis by the chicken monocytes was observed when the Z-average lyosol particle size was ~ 50 nm, which was achieved with a turpentine dry matter ratio of 1:200 during the synthesis.