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Application of Time-Resolved Fluorescence Microscopy for Enhancing the Selectivity of Fluorogenic Dyes of the Arylidene–Imidazolone Series toward the Endoplasmic Reticulum
Objective: A number of previously synthesized fluorogenic arylidene-imidazolones, which predominantly stain the endoplasmic reticulum (ER) of living cells, were studied by time-resolved fluorescence microscopy. It was suggested that the use of fluorescence microscopy of this type can enhance the sel...
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| Published in: | Russian journal of bioorganic chemistry 2024-10, Vol.50 (5), p.1896-1903 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 1903 |
| container_issue | 5 |
| container_start_page | 1896 |
| container_title | Russian journal of bioorganic chemistry |
| container_volume | 50 |
| creator | Gilvanov, A. R. Smirnov, A. Yu Krasnova, S. A. Solovyev, I. D. Savitsky, A. P. Bogdanova, Yu. A. Baranov, M. S. |
| description | Objective:
A number of previously synthesized fluorogenic arylidene-imidazolones, which predominantly stain the endoplasmic reticulum (ER) of living cells, were studied by time-resolved fluorescence microscopy. It was suggested that the use of fluorescence microscopy of this type can enhance the selectivity of ER staining.
Methods:
The lifetimes of arylidene-imidazolone compounds in set of solvents with different polarity were measured with time-correlated single photon counting spectroscopy. Live HeLa Kyoto cells were stained with studied dyes and analyzed with time-resolved fluorescence microscopy.
Results and discussion:
It was found that most of the studied compounds show bi- or triexponential fluorescence decay patterns in solutions. In live cell culture dye (
I
) showed a monoexponential decay pattern, while dyes (
II–IV
) were better fitted by a biexponential function. Dyes (
I
), (
III
), and (
IV
) stained both ER and adiposomes, while dye (
II
) stained only ER.
Conclusions:
It is shown that under FLIM conditions discriminative filtering of cellular organelles stained with studied fluorogenic dyes is possible and applicable if the difference of mean amplitude-weighted lifetime is more than 0.1 ns, thus increasing the selectivity of ER staining in live cells. |
| doi_str_mv | 10.1134/S1068162024050315 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_3115023632</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3115023632</sourcerecordid><originalsourceid>FETCH-LOGICAL-c198t-890354c0c2ac8cfb8a911d17ec5bf5e64d7c7d1eb61277e5e398d0578821ab6b3</originalsourceid><addsrcrecordid>eNp1kcFq3DAQhk1JoUnaB-hN0LMTjbWytcdls0kXNgSSLfRmZGm8UZAlV7I3uKe8Q54jL5UniZ0t9FB6GjHzff-AJkm-Aj0DYLPzO6C5gDyj2YxyyoB_SI4hpyJljP48Gt_jOJ3mn5KTGB8oBUq5OE5eFm1rjZKd8Y74mmxNg-ktRm_3qMml7X3AqNApJNdGBR-VbwdS-0BW7l46ZdyOdPdI7tCi6szedMMU8y76HTqjyMWAcepN2CIM1mh0-Pr0vG6Mlr-99W7Sgxmpzj_KoN_JldO-tTI2Y8Itdkb1tm8-Jx9raSN--VNPkx-Xq-3ye7q5uVovF5tUwVx0qZhTxmeKqkwqoepKyDmAhgIVr2qO-UwXqtCAVQ5ZUSBHNhea8kKIDGSVV-w0-XbIbYP_1WPsygffBzeuLBkApxnLWTZScKCmf4kB67INppFhKIGW01XKf64yOtnBiSPrdhj-Jv9fegO_CZNO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3115023632</pqid></control><display><type>article</type><title>Application of Time-Resolved Fluorescence Microscopy for Enhancing the Selectivity of Fluorogenic Dyes of the Arylidene–Imidazolone Series toward the Endoplasmic Reticulum</title><source>Springer Link</source><creator>Gilvanov, A. R. ; Smirnov, A. Yu ; Krasnova, S. A. ; Solovyev, I. D. ; Savitsky, A. P. ; Bogdanova, Yu. A. ; Baranov, M. S.</creator><creatorcontrib>Gilvanov, A. R. ; Smirnov, A. Yu ; Krasnova, S. A. ; Solovyev, I. D. ; Savitsky, A. P. ; Bogdanova, Yu. A. ; Baranov, M. S.</creatorcontrib><description>Objective:
A number of previously synthesized fluorogenic arylidene-imidazolones, which predominantly stain the endoplasmic reticulum (ER) of living cells, were studied by time-resolved fluorescence microscopy. It was suggested that the use of fluorescence microscopy of this type can enhance the selectivity of ER staining.
Methods:
The lifetimes of arylidene-imidazolone compounds in set of solvents with different polarity were measured with time-correlated single photon counting spectroscopy. Live HeLa Kyoto cells were stained with studied dyes and analyzed with time-resolved fluorescence microscopy.
Results and discussion:
It was found that most of the studied compounds show bi- or triexponential fluorescence decay patterns in solutions. In live cell culture dye (
I
) showed a monoexponential decay pattern, while dyes (
II–IV
) were better fitted by a biexponential function. Dyes (
I
), (
III
), and (
IV
) stained both ER and adiposomes, while dye (
II
) stained only ER.
Conclusions:
It is shown that under FLIM conditions discriminative filtering of cellular organelles stained with studied fluorogenic dyes is possible and applicable if the difference of mean amplitude-weighted lifetime is more than 0.1 ns, thus increasing the selectivity of ER staining in live cells.</description><identifier>ISSN: 1068-1620</identifier><identifier>EISSN: 1608-330X</identifier><identifier>DOI: 10.1134/S1068162024050315</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Decay ; Dyes ; Endoplasmic reticulum ; Fluorescence ; Heterocyclic compounds ; Life Sciences ; Microscopy ; Organelles ; Organic Chemistry ; Staining ; Time correlation functions ; Time measurement</subject><ispartof>Russian journal of bioorganic chemistry, 2024-10, Vol.50 (5), p.1896-1903</ispartof><rights>Pleiades Publishing, Ltd. 2024</rights><rights>Pleiades Publishing, Ltd. 2024.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c198t-890354c0c2ac8cfb8a911d17ec5bf5e64d7c7d1eb61277e5e398d0578821ab6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Gilvanov, A. R.</creatorcontrib><creatorcontrib>Smirnov, A. Yu</creatorcontrib><creatorcontrib>Krasnova, S. A.</creatorcontrib><creatorcontrib>Solovyev, I. D.</creatorcontrib><creatorcontrib>Savitsky, A. P.</creatorcontrib><creatorcontrib>Bogdanova, Yu. A.</creatorcontrib><creatorcontrib>Baranov, M. S.</creatorcontrib><title>Application of Time-Resolved Fluorescence Microscopy for Enhancing the Selectivity of Fluorogenic Dyes of the Arylidene–Imidazolone Series toward the Endoplasmic Reticulum</title><title>Russian journal of bioorganic chemistry</title><addtitle>Russ J Bioorg Chem</addtitle><description>Objective:
A number of previously synthesized fluorogenic arylidene-imidazolones, which predominantly stain the endoplasmic reticulum (ER) of living cells, were studied by time-resolved fluorescence microscopy. It was suggested that the use of fluorescence microscopy of this type can enhance the selectivity of ER staining.
Methods:
The lifetimes of arylidene-imidazolone compounds in set of solvents with different polarity were measured with time-correlated single photon counting spectroscopy. Live HeLa Kyoto cells were stained with studied dyes and analyzed with time-resolved fluorescence microscopy.
Results and discussion:
It was found that most of the studied compounds show bi- or triexponential fluorescence decay patterns in solutions. In live cell culture dye (
I
) showed a monoexponential decay pattern, while dyes (
II–IV
) were better fitted by a biexponential function. Dyes (
I
), (
III
), and (
IV
) stained both ER and adiposomes, while dye (
II
) stained only ER.
Conclusions:
It is shown that under FLIM conditions discriminative filtering of cellular organelles stained with studied fluorogenic dyes is possible and applicable if the difference of mean amplitude-weighted lifetime is more than 0.1 ns, thus increasing the selectivity of ER staining in live cells.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Decay</subject><subject>Dyes</subject><subject>Endoplasmic reticulum</subject><subject>Fluorescence</subject><subject>Heterocyclic compounds</subject><subject>Life Sciences</subject><subject>Microscopy</subject><subject>Organelles</subject><subject>Organic Chemistry</subject><subject>Staining</subject><subject>Time correlation functions</subject><subject>Time measurement</subject><issn>1068-1620</issn><issn>1608-330X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1kcFq3DAQhk1JoUnaB-hN0LMTjbWytcdls0kXNgSSLfRmZGm8UZAlV7I3uKe8Q54jL5UniZ0t9FB6GjHzff-AJkm-Aj0DYLPzO6C5gDyj2YxyyoB_SI4hpyJljP48Gt_jOJ3mn5KTGB8oBUq5OE5eFm1rjZKd8Y74mmxNg-ktRm_3qMml7X3AqNApJNdGBR-VbwdS-0BW7l46ZdyOdPdI7tCi6szedMMU8y76HTqjyMWAcepN2CIM1mh0-Pr0vG6Mlr-99W7Sgxmpzj_KoN_JldO-tTI2Y8Itdkb1tm8-Jx9raSN--VNPkx-Xq-3ye7q5uVovF5tUwVx0qZhTxmeKqkwqoepKyDmAhgIVr2qO-UwXqtCAVQ5ZUSBHNhea8kKIDGSVV-w0-XbIbYP_1WPsygffBzeuLBkApxnLWTZScKCmf4kB67INppFhKIGW01XKf64yOtnBiSPrdhj-Jv9fegO_CZNO</recordid><startdate>20241001</startdate><enddate>20241001</enddate><creator>Gilvanov, A. R.</creator><creator>Smirnov, A. Yu</creator><creator>Krasnova, S. A.</creator><creator>Solovyev, I. D.</creator><creator>Savitsky, A. P.</creator><creator>Bogdanova, Yu. A.</creator><creator>Baranov, M. S.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20241001</creationdate><title>Application of Time-Resolved Fluorescence Microscopy for Enhancing the Selectivity of Fluorogenic Dyes of the Arylidene–Imidazolone Series toward the Endoplasmic Reticulum</title><author>Gilvanov, A. R. ; Smirnov, A. Yu ; Krasnova, S. A. ; Solovyev, I. D. ; Savitsky, A. P. ; Bogdanova, Yu. A. ; Baranov, M. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c198t-890354c0c2ac8cfb8a911d17ec5bf5e64d7c7d1eb61277e5e398d0578821ab6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Decay</topic><topic>Dyes</topic><topic>Endoplasmic reticulum</topic><topic>Fluorescence</topic><topic>Heterocyclic compounds</topic><topic>Life Sciences</topic><topic>Microscopy</topic><topic>Organelles</topic><topic>Organic Chemistry</topic><topic>Staining</topic><topic>Time correlation functions</topic><topic>Time measurement</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gilvanov, A. R.</creatorcontrib><creatorcontrib>Smirnov, A. Yu</creatorcontrib><creatorcontrib>Krasnova, S. A.</creatorcontrib><creatorcontrib>Solovyev, I. D.</creatorcontrib><creatorcontrib>Savitsky, A. P.</creatorcontrib><creatorcontrib>Bogdanova, Yu. A.</creatorcontrib><creatorcontrib>Baranov, M. S.</creatorcontrib><collection>CrossRef</collection><jtitle>Russian journal of bioorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gilvanov, A. R.</au><au>Smirnov, A. Yu</au><au>Krasnova, S. A.</au><au>Solovyev, I. D.</au><au>Savitsky, A. P.</au><au>Bogdanova, Yu. A.</au><au>Baranov, M. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of Time-Resolved Fluorescence Microscopy for Enhancing the Selectivity of Fluorogenic Dyes of the Arylidene–Imidazolone Series toward the Endoplasmic Reticulum</atitle><jtitle>Russian journal of bioorganic chemistry</jtitle><stitle>Russ J Bioorg Chem</stitle><date>2024-10-01</date><risdate>2024</risdate><volume>50</volume><issue>5</issue><spage>1896</spage><epage>1903</epage><pages>1896-1903</pages><issn>1068-1620</issn><eissn>1608-330X</eissn><abstract>Objective:
A number of previously synthesized fluorogenic arylidene-imidazolones, which predominantly stain the endoplasmic reticulum (ER) of living cells, were studied by time-resolved fluorescence microscopy. It was suggested that the use of fluorescence microscopy of this type can enhance the selectivity of ER staining.
Methods:
The lifetimes of arylidene-imidazolone compounds in set of solvents with different polarity were measured with time-correlated single photon counting spectroscopy. Live HeLa Kyoto cells were stained with studied dyes and analyzed with time-resolved fluorescence microscopy.
Results and discussion:
It was found that most of the studied compounds show bi- or triexponential fluorescence decay patterns in solutions. In live cell culture dye (
I
) showed a monoexponential decay pattern, while dyes (
II–IV
) were better fitted by a biexponential function. Dyes (
I
), (
III
), and (
IV
) stained both ER and adiposomes, while dye (
II
) stained only ER.
Conclusions:
It is shown that under FLIM conditions discriminative filtering of cellular organelles stained with studied fluorogenic dyes is possible and applicable if the difference of mean amplitude-weighted lifetime is more than 0.1 ns, thus increasing the selectivity of ER staining in live cells.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1068162024050315</doi><tpages>8</tpages></addata></record> |
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| issn | 1068-1620 1608-330X |
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| subjects | Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Decay Dyes Endoplasmic reticulum Fluorescence Heterocyclic compounds Life Sciences Microscopy Organelles Organic Chemistry Staining Time correlation functions Time measurement |
| title | Application of Time-Resolved Fluorescence Microscopy for Enhancing the Selectivity of Fluorogenic Dyes of the Arylidene–Imidazolone Series toward the Endoplasmic Reticulum |
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