Pixelation with Concentration‐Encoded Effective Photons for Quantitative Molecular Optical Sectioning Microscopy (Laser Photonics Rev. 18(10)/2024)

Quantitative Microscopy Haohua Tu and co‐workers developed a simple universal tool, termed “Pixelation with Concentration‐encoded Effective Photons” (PCEP), to address critical concerns on the reproducibility in light microscopy (see article number 2400031). PCEP unifies the unit of pixelated measur...

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Bibliographic Details
Published in:Laser & photonics reviews 2024-10, Vol.18 (10), p.n/a
Main Authors: Wang, Geng, Iyer, Rishyashring R., Sorrells, Janet E., Aksamitiene, Edita, Chaney, Eric J., Renteria, Carlos A., Park, Jaena, Shi, Jindou, Sun, Yi, Boppart, Stephen A., Tu, Haohua
Format: Article
Language:English
Subjects:
Coding
Optical microscopy
Optical sectioning
Photons
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Summary:Quantitative Microscopy Haohua Tu and co‐workers developed a simple universal tool, termed “Pixelation with Concentration‐encoded Effective Photons” (PCEP), to address critical concerns on the reproducibility in light microscopy (see article number 2400031). PCEP unifies the unit of pixelated measurement for image pixel representation using the “effective photon” and enables performance benchmarking of different microscopes, such as laser‐scanning/wide‐field microscopy. This technology is beneficial in label‐free and labeled fluorescence microscopy in clinical and biological applications.
ISSN:1863-8880
1863-8899
DOI:10.1002/lpor.202470061