Production of polyclonal anti-human follicle stimulating hormone (hFSH) in Balb/C mice for standardizing an immunoradiometric assay for hFSH

• Published in: Journal of radioanalytical and nuclear chemistry 2024-11, Vol.333 (11), p.5263-5276
• Main Authors: Ebeid, N. H., Shafik, H. M., Mehany, N. L.
• Format: Article
• Language: English
• Subjects: Assaying; Chemistry; Chemistry and Materials Science; Correlation coefficients; Diagnostic Radiology; Hadrons; Heavy Ions; Hypothalamus; Inorganic Chemistry; Nuclear Chemistry; Nuclear Physics; Physical Chemistry; Radiochemistry
• ISSN: 0236-5731; 1588-2780
• DOI: 10.1007/s10967-024-09647-8

This study aimed to produce polyclonal antibodies of human follicle-stimulating hormone (anti-hFSH) in order to establish an immunoradiometric assay (IRMA). The anti-hFSH was tested using the prepared 125 I-hFSH tracer. The 125 I-hFSH tracer was carried out using the lactoperoxidase method, resulting in a radiochemical yield of 87.6% and a specific activity of 136 μCi/μg. The anti-hFSH produced gave highest displacement percentages (80.3, 79.8 and 71.2%) and a successful immunoresponse. hFSH-IRMA system was developed using 125 I-MoAb-hFSH tracer (DIA Source). The results show a precise, stable and accurate measurements of hFSH-IRMA with a sensitivity of 0.15 mIU/ml. The coefficient variation for intra-assay is less than ± 10% and for inter-assay, it is less than ± 15%. Additionally, the liner correlation coefficient r is equal to 0.999. This technique can be utilized as a helpful diagnostic tool to study fertility and diseases of the hypothalamus-pituitary–gonadal axis in humans.