Novel Biosynthesis and Evaluation Cytotoxic Effect of Tellurium Nano-particle on Breast Cancer Using Co-culture of Spirulina and Lentinula edodes

• Published in: BioNanoScience 2024-11, Vol.14 (4), p.4624-4632
• Main Authors: Reza, Mahdieh Ameri Shah, Rasouli, Alireza, Movahhed, Tahereh Komeili
• Format: Article
• Language: English
• Subjects: Algae; Biocompatibility; Biological and Medical Physics; Biomaterials; Biomedical materials; Biophysics; Biosynthesis; Cancer; Cell culture; Circuits and Systems; Cytotoxicity; Engineering; Field emission microscopy; Field emission spectroscopy; Fourier transforms; Fungi; Fungiculture; Infrared spectroscopy; Nanoparticles; Nanotechnology; Scanning electron microscopy; Shape effects; Side effects; Tellurium; Toxicity; Tumor cell lines
• ISSN: 2191-1630; 2191-1649
• DOI: 10.1007/s12668-024-01592-w

Bio-based nanoparticles (NPs) production has been an alternative method for preparing NPs for pharmaceutical and biomedical applications. This study aims to evaluate the production of tellurium nanoparticles (TeNPs) using the co-culture of algae and fungi and finally to investigate the cytotoxicity of NPs on cancer cell lines MDA-MB-468 and healthy cell line HFF. The production of TeNPs was done using the co-culture of algae and fungi. Using the Tyndall effect, Fourier transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FESEM), NPs were evaluated. Finally, using the MTT assay, the toxicity of the NPs produced on MDA-MB-468 and HFF was investigated. TeNPs were synthesized using co-culture, and the average size of NPs was 20 to 30 nm. They have a spherical shape and superior monodispersity. It also found that NPs have a time—and concentration-dependent inhibitory effect on cancer cells, while they do not affect healthy cells. This research is the first report on the co-cultivation of two different strains of algae and fungi to synthesize NPs. Many studies are needed in the future to determine the characteristics of this method. Still, considering the characteristics of both organisms used, it can be said that NPs synthesized have improved stability and properties. The use of NPs in the fight against cancer has many features, including increasing effectiveness and reducing side effects, and can replace the current risky methods in the future; for this reason, developing new methods of NPs synthesis is very important.