Optimizing the combination of SiO2NPs and CeO2NPs on relative growth rate of Fragaria × ananassa plantlets cultured in vitro

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2024-11, Vol.159 (2), p.43, Article 43
• Main Authors: Cuong, Do Manh, Mai, Nguyen Thi Nhu, Luan, Vu Quoc, Tung, Hoang Thanh, The Vinh, Bui Van, Thuy, Nguyen Thi Thanh, Nguyen, Phan Le Ha, Hiep, Phan Phuoc Minh, Dang, Hoang Hai, Van Hoang, Cao, Vinh, Nguyen Quang, Nhut, Duong Tan
• Format: Article
• Language: English
• Subjects: Absorption; Acetic acid; Antioxidants; Biomedical and Life Sciences; Cerium; Cerium oxides; Combined treatment; Crop yield; Developmental stages; Enzymes; Fragaria ananassa; Fruit crops; Growth rate; In vitro methods and tests; Leaves; Life Sciences; Minerals; Nanoparticles; Naphthalene; Optimization; Original Article; Plant Genetics and Genomics; Plant growth; Plant layout; Plant Pathology; Plant Physiology; Plant Sciences; Plantlets; Plants (botany); Response surface methodology; Shoots; Silicon dioxide; Stimulants
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-024-02902-6

Fragaria × ananassa is one of the fruit crops with high economic value, but due to its thin and long stem structure, it is easily affected by various stresses when being transferred from in vitro to ex vitro conditions. Therefore, it is very important to enhance the relative growth rate (RGR) right from the in vitro stage which is in proportion to the vigorousness of the plant. Nanoparticles are usually known as stimulants in agriculture that promote plant growth and development. In this study, in vitro Fragaria × ananassa shoots transferred on Murashige and Skoog (MS) medium supplemented with 0.02 mg/L α-naphthalene acetic acid (NAA) were used as control. Silicon dioxide nanoparticles (SiO 2 NPs) and cerium oxide nanoparticles (CeO 2 NPs) at different concentrations (0, 0.5, 1.0, 1.5 and 2.0 mg/L) were separately added to the similar MS medium in the plantlet formation stage to evaluate their effects on RGR of in vitro Fragaria × ananassa plantlets through their impacts on minerals absorption (K, Ca, Mg) and antioxidant enzymes activity. The results showed that the medium supplemented with 1.0 mg/L SiO 2 NPs gave the highest minerals absorption while the medium with 1.0–1.5 mg/L CeO 2 NPs created the most favorable conditions for antioxidant enzymes activity (SOD, CAT, APX) compared to treatments supplemented with SiO 2 NPs or CeO 2 NPs at other concentrations and the control. Specifically, plant height and leaf canopy area were recorded highest in the medium supplemented with 1.0 mg/L SiO 2 NPs, and the medium supplemented with 1.5 mg/L CeO 2 NPs gave the highest values of leaf petiole diameter, number of roots, root length. Then, the response surface methodology (RSM) was used to optimize the combination of SiO 2 NPs and CeO 2 NPs at appropriate concentrations with the aim of finding the most optimal concentrations for the RGR of plantlet. Accordingly, the most optimal concentrations are 1.172 mg/L SiO 2 NPs and 1.654 mg/L CeO 2 NPs. The combination of these optimal concentrations in the plantlet formation stage gave plantlet’s RGR (0.596 mg/mg/week) superior to the highest RGR obtained from the supplementation with 1.0 mg/L SiO 2 NPs or 1.5 mg/L CeO 2 NPs (0.53 or 0.56 mg/mg/week, respectively). As a result, shoots-derived-plantlets in the combined treatment were taller and had more vigorous growth. This study can be useful in commercial strawberry production, increasing plant growth, vigorousness and resistance right from the in vitro stage, thereby i