The relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia

OBJECTIVES: To investigate the relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia. MATERIAL AND METHODS: Real-time PCR (qRT-PCR) was used to detect the hsa_circ_0051326 expression level. Enzyme-linked immunosorbent assay (ELISA), qRT-PCR, and weste...

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Bibliographic Details
Published in:Ginekologia polska 2024-01, Vol.95 (10), p.803-809
Main Authors: Wang, Li, Wang, Xue, Chen, Xiaoju, Wu, Dongcai, Cen, Hui, Mao, Dongrui, Mo, Yuqiao, Zheng, Linmei
Format: Article
Language:English
Subjects:
Antigens
Biomarkers
Blood pressure
Enzymes
Fetuses
Hospitals
Leukocytes
Medical research
Obstetrics
Preeclampsia
Pregnancy
Proteins
Reagents
Womens health
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Summary:OBJECTIVES: To investigate the relationship between hsa_circ_0051326 and HLA-G expression in the blood of patients with pre-eclampsia. MATERIAL AND METHODS: Real-time PCR (qRT-PCR) was used to detect the hsa_circ_0051326 expression level. Enzyme-linked immunosorbent assay (ELISA), qRT-PCR, and western blotting were used to detect HLA-G expression in blood in 50 patients with pre-eclampsia and 50 normal pregnant women. RESULTS: HLA-G protein expression level was decreased significantly in the blood of patients with pre-eclampsia. hsa_circ_0051326 and HLA-G mRNA in blood were decreased significantly in the pre-eclampsia patients compared with normal pregnant women. There was a positive correlation between the expression of serum hsa_circ_0051326 with HLA-G mRNA. CONCLUSIONS: Serum hsa_circ_0051326 was a new diagnostic biomarker for pre-eclampsia with equivalent efficiency of HLA-G. Maternal serum hsa_circ_0051326 level may pre-diagnose potentially pre-eclampsia before delivery.
ISSN:0017-0011
2543-6767
DOI:10.5603/GP.a2021.0194