Lipase production from Bacillus licheniformis MTCC8725 by comparison of melon wastes and olive oil under solid-state fermentation

• Main Authors: Karunya, Dharmalingam, Arulvel, Ramaswamy
• Format: Conference Proceeding
• Language: English
• Subjects: Enzymes; Fermentation; Inoculum; Lipase; Moisture content; Olive oil; Solid state; Wastes
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0233945

This study aimed to assess the performance of employing olive oil and melon wastes as substrates for Bacillus licheniformis MTCC8725 lipase production. Research Tools and Procedures: An effective component of the method was the use of the bacterial strains in this investigation. The goal of the process was to cultivate Bacillus licheniformis, a microbe (MTCC8725). Olive oil and melon byproducts served as the primary supports. The following procedures were carried out to manufacture lipase: substrate preparation, inoculum preparation, solid-state fermentation, enzyme extraction, and lipase testing. The results showed that an independent sample T-test yielded a value of 0.03 (p>0.05), indicating statistical significance, and that the sample size was 8 with 80% G power. At ideal culture conditions of 40°C, pH 5.5, and 70% moisture content (v/w), the lipase activity in melon wastes was 14.57 U/g, whereas in olive oil it was 12.24 U/mL. Conclusion: By adjusting several factors, it was demonstrated that the yield was higher in melon wastes compared to olive oil while producing lipase enzyme.