Accelerating Diverse Cell-Based Therapies Through Scalable Design

Augmenting cells with novel, genetically encoded functions will support therapies that expand beyond natural capacity for immune surveillance and tissue regeneration. However, engineering cells at scale with transgenic cargoes remains a challenge in realizing the potential of cell-based therapies. I...

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Bibliographic Details
Published in:Annual review of chemical and biomolecular engineering 2024-01, Vol.15 (1), p.267-292
Main Authors: Peterman, Emma L, Ploessl, Deon S, Galloway, Kate E
Format: Article
Language:English
Subjects:
biomanufacturing
Cargo capacity
cell-based therapies
cellular engineering
Genetic code
genome editing
Immune system
Immunosurveillance
Regeneration (physiology)
Stem cells
synthetic biology
Tissue engineering
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Summary:Augmenting cells with novel, genetically encoded functions will support therapies that expand beyond natural capacity for immune surveillance and tissue regeneration. However, engineering cells at scale with transgenic cargoes remains a challenge in realizing the potential of cell-based therapies. In this review, we introduce a range of applications for engineering primary cells and stem cells for cell-based therapies. We highlight tools and advances that have launched mammalian cell engineering from bioproduction to precision editing of therapeutically relevant cells. Additionally, we examine how transgenesis methods and genetic cargo designs can be tailored for performance. Altogether, we offer a vision for accelerating the translation of innovative cell-based therapies by harnessing diverse cell types, integrating the expanding array of synthetic biology tools, and building cellular tools through advanced genome writing techniques.
ISSN:1947-5438
1947-5446
1947-5446
DOI:10.1146/annurev-chembioeng-100722-121610