Chemoprotective effect of arbutin on azoxymethaneinduced aberrant crypt foci in rat colon via modulation of PCNA/Bax protein

Arbutin is utilized in traditional remedies to cure numerous syndromes because of its anti-microbial, antioxidant, and antiinflammatory properties. This study aimed to evaluate chemopreventive effects of arbutin on azoxymethane (AOM)-induced colon aberrant crypt foci (ACF) in rats. Five groups of ra...

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Bibliographic Details
Published in:Brazilian journal of medical and biological research 2024-01, Vol.57, p.1
Main Authors: Ahmed, K A, Shareef, S H, Faraj, T A, Abdulla, M A, Najmaldin, S K, Agha, N F S, Kheder, R K
Format: Article
Language:English
Subjects:
5-Fluorouracil
Anti-inflammatory agents
Antioxidants
Azoxymethane
BAX protein
Colon cancer
Colorectal carcinoma
Goblet cells
PCNA protein
Proliferating cell nuclear antigen
Proteins
Superoxide dismutase
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Summary:Arbutin is utilized in traditional remedies to cure numerous syndromes because of its anti-microbial, antioxidant, and antiinflammatory properties. This study aimed to evaluate chemopreventive effects of arbutin on azoxymethane (AOM)-induced colon aberrant crypt foci (ACF) in rats. Five groups of rats were used: normal control group (rats injected hypodermically with sterile phosphate-buffered saline once per week for two weeks) and groups 2–5, which were subcutaneously inoculated with 15 mg/kg AOM once a week for two weeks. AOM control and 5-fluorouracil (5-FU) control groups were fed 10% Tween orally daily for 8 weeks using a feeding tube. The treated groups were fed 30 and 60 mg/kg arbutin every day for 2 months. ACF from the AOM control group had aberrant nuclei in addition to multilayered cells and an absence of goblet cells. The negative control group displayed spherical cells and nuclei in basal positions. Histological examination revealed a reduced number of AFC cells from colon tissues of the 5-FU reference group. Arbutin-fed animals showed down-regulation of proliferating cell nuclear antigen (PCNA) and up-regulation of Bax protein compared to AOM control. Rats fed with arbutin displayed a significant increase of superoxide dismutase (SOD) and catalase (CAT) activities in colon tissue homogenates compared to the AOM control group. In conclusion, arbutin showed therapeutic effects against colorectal cancer, explained by its ability to significantly decrease ACF, down-regulate PCNA protein, and up-regulate Bax protein. In addition, arbutin significantly increased SOD and CAT, and decreased malondialdehyde (MDA) levels, which might be due to its anti-proliferative and antioxidant properties.
ISSN:0100-879X
1678-4510
DOI:10.1590/1414-431X2024e13306