An Immunohistochemical Study of Na+/I− Symporter in Human Thyroid Tissues and Salivary Gland Tissues

The human Na+/I− symporter (hNIS) is the plasma membrane protein that mediates active iodide uptake into several tissues, such as the thyroid and salivary glands. To study the distribution and cellular localization of the hNIS protein, we have generated a polyclonal antibody that could detect the hN...

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Bibliographic Details
Published in:Endocrinology (Philadelphia) 1998-10, Vol.139 (10), p.4416-4419
Main Authors: Jhiang, Sissy M, Cho, Je-Yoel, Ryu, Kwon-Yul, DeYoung, Barry R, Smanik, Patricia A, McGaughy, Violeta R, Fischer, Andrew H, Mazzaferri, Ernest L
Format: Article
Language:English
Subjects:
Acinar cells
Immunohistochemistry
Iodides
Localization
Membranes
Polyclonal antibodies
Proteins
Salivary gland
Salivary glands
Thyrocytes
Thyroid
Thyroid carcinoma
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Summary:The human Na+/I− symporter (hNIS) is the plasma membrane protein that mediates active iodide uptake into several tissues, such as the thyroid and salivary glands. To study the distribution and cellular localization of the hNIS protein, we have generated a polyclonal antibody that could detect the hNIS protein by immunohistochemical staining on tissue sections. In normal thyroids, hNIS expression is heterogeneous, and it is only detected in sporadic thyrocytes of a given follicle. The hNIS protein was not detected in thyroid carcinomas, yet it was detected in the majority of thyrocytes in Graves’ thyroids. In salivary glands, hNIS protein was not detected in acinar cells, but it was detected in ductal cells. The hNIS proteins are clustered in the basal and lateral membranes in cells stained positive for hNIS.
ISSN:0013-7227
1945-7170
DOI:10.1210/endo.139.10.6329