Cloning and Characterization of the Chicken Thyrotropin-Releasing Hormone Receptor

Abstract We report on the cloning of the full-length complementary DNA for the chicken TRH receptor. Although the TRH receptor has been cloned from several mammalian species, this is the first report from another vertebrate class. The ligand binding pocket, which is situated in the transmembrane hel...

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Bibliographic Details
Published in:Endocrinology (Philadelphia) 1998-08, Vol.139 (8), p.3390-3398
Main Authors: Sun, Yuh-Man, Millar, Robert P., Ho, Hao, Gershengorn, Marvin C., Illing, Nicola
Format: Article
Language:English
Subjects:
Binding
Chickens
Cloning
Intracellular signalling
Poultry
Receptor mechanisms
Receptors
Structure-function relationships
Thyroid-stimulating hormone
Thyrotropin-releasing hormone
Vertebrates
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Summary:Abstract We report on the cloning of the full-length complementary DNA for the chicken TRH receptor. Although the TRH receptor has been cloned from several mammalian species, this is the first report from another vertebrate class. The ligand binding pocket, which is situated in the transmembrane helixes of the mouse and rat TRH receptors, is completely conserved in the chicken receptor. Pharmacological studies (receptor binding and signaling) employing several TRH analogs revealed that there are no significant differences between the chicken and mouse receptors. These findings show that there have been considerable evolutionary constraints on TRH receptor structure and function. Several truncated forms of the chicken TRH receptor that appear to retain a part of an intron and are truncated in the putative third intracellular loop were also cloned, but were nonfunctional. This study provides a useful tool for further studies on the roles of TRH in avian growth and TSH regulation.
ISSN:0013-7227
1945-7170
DOI:10.1210/endo.139.8.6133