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Distinct Subcellular Localization of Transiently Expressed Types 1 and 2 Iodothyronine Deiodinases as Determined by Immunofluorescence Confocal Microscopy
We compared the subcellular localization of FLAG-epitope tagged Types 1 and 2 deiodinases (D1 and D2) transiently expressed in human embryonic kidney (HEK-293) and mouse neuroblastoma (NB2A) cells. D2 is an integral membrane protein based on resistance to extraction at pH 11 with the NH2 terminus in...
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| Published in: | Endocrinology (Philadelphia) 2000-11, Vol.141 (11), p.4309-4312 |
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| Main Authors: | , , , , |
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| container_end_page | 4312 |
| container_issue | 11 |
| container_start_page | 4309 |
| container_title | Endocrinology (Philadelphia) |
| container_volume | 141 |
| creator | Baqui, Munira M. A Gereben, Balazs Harney, John W Larsen, P. Reed Bianco, Antonio C |
| description | We compared the subcellular localization of FLAG-epitope tagged Types 1
and 2 deiodinases (D1 and D2) transiently expressed in human embryonic
kidney (HEK-293) and mouse neuroblastoma (NB2A) cells. D2 is an
integral membrane protein based on resistance to extraction at pH 11
with the NH2 terminus in the endoplasmic reticulum (ER).
Immunofluorescence confocal microscopy using anti-FLAG and
anti-GRP78/BiP antibodies showed the FLAG-D1 signal was found in the
periphery of the cells and not co-localized with the ER specific marker
GRP78/BiP. On the other hand, FLAG-D2 protein was found in the ER
co-localized with the GRP78/BiP protein. These differential
distribution patterns indicate subcellular sorting of D1 and D2 is
determined by intrinsic protein sequence and can explain the ready
access of D2-generated T3 to the nucleus. |
| doi_str_mv | 10.1210/endo.141.11.7872 |
| format | article |
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and 2 deiodinases (D1 and D2) transiently expressed in human embryonic
kidney (HEK-293) and mouse neuroblastoma (NB2A) cells. D2 is an
integral membrane protein based on resistance to extraction at pH 11
with the NH2 terminus in the endoplasmic reticulum (ER).
Immunofluorescence confocal microscopy using anti-FLAG and
anti-GRP78/BiP antibodies showed the FLAG-D1 signal was found in the
periphery of the cells and not co-localized with the ER specific marker
GRP78/BiP. On the other hand, FLAG-D2 protein was found in the ER
co-localized with the GRP78/BiP protein. These differential
distribution patterns indicate subcellular sorting of D1 and D2 is
determined by intrinsic protein sequence and can explain the ready
access of D2-generated T3 to the nucleus.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/endo.141.11.7872</identifier><language>eng</language><publisher>Washington: Endocrine Society</publisher><subject>Amino acid sequence ; BiP protein ; Confocal microscopy ; D2 protein ; Differential distribution ; Endoplasmic reticulum ; Epitopes ; Flags ; Immunofluorescence ; Localization ; Microscopy ; Neuroblastoma ; Protein transport ; Proteins</subject><ispartof>Endocrinology (Philadelphia), 2000-11, Vol.141 (11), p.4309-4312</ispartof><rights>Copyright © 2000 by the Endocrine Society 2000</rights><rights>Copyright © 2000 by the Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3102-e689873aaa8a7037f4bda520a7efa0ca327e34f272f6845d08e2af89f3a55b4b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Baqui, Munira M. A</creatorcontrib><creatorcontrib>Gereben, Balazs</creatorcontrib><creatorcontrib>Harney, John W</creatorcontrib><creatorcontrib>Larsen, P. Reed</creatorcontrib><creatorcontrib>Bianco, Antonio C</creatorcontrib><title>Distinct Subcellular Localization of Transiently Expressed Types 1 and 2 Iodothyronine Deiodinases as Determined by Immunofluorescence Confocal Microscopy</title><title>Endocrinology (Philadelphia)</title><description>We compared the subcellular localization of FLAG-epitope tagged Types 1
and 2 deiodinases (D1 and D2) transiently expressed in human embryonic
kidney (HEK-293) and mouse neuroblastoma (NB2A) cells. D2 is an
integral membrane protein based on resistance to extraction at pH 11
with the NH2 terminus in the endoplasmic reticulum (ER).
Immunofluorescence confocal microscopy using anti-FLAG and
anti-GRP78/BiP antibodies showed the FLAG-D1 signal was found in the
periphery of the cells and not co-localized with the ER specific marker
GRP78/BiP. On the other hand, FLAG-D2 protein was found in the ER
co-localized with the GRP78/BiP protein. These differential
distribution patterns indicate subcellular sorting of D1 and D2 is
determined by intrinsic protein sequence and can explain the ready
access of D2-generated T3 to the nucleus.</description><subject>Amino acid sequence</subject><subject>BiP protein</subject><subject>Confocal microscopy</subject><subject>D2 protein</subject><subject>Differential distribution</subject><subject>Endoplasmic reticulum</subject><subject>Epitopes</subject><subject>Flags</subject><subject>Immunofluorescence</subject><subject>Localization</subject><subject>Microscopy</subject><subject>Neuroblastoma</subject><subject>Protein transport</subject><subject>Proteins</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqNkU9r3DAQxUVpodu09x4FORZv9M8r77FsknZhQw_dnsVYHlEFr-RINtT5KP20kbuBngI5DcP83sxjHiGfOVtzwdkVhi6uueJrzte60eINWfGtqivNNXtLVoxxWWkh9HvyIef70iql5Ir8vfZ59MGO9OfUWuz7qYdED9FC7x9h9DHQ6OgxQcgew9jP9ObPkDBn7OhxHjBTTiF0VNB97OL4e04x-ID0Gn3sfIBcCMilHTGdyqCj7Uz3p9MUouunWDZZDBbpLga3HKV33qaYbRzmj-Sdgz7jp-d6QX7d3hx336vDj2_73ddDZSVnosJNs220BIAGNJPaqbaDWjDQ6IBZkEKjVE5o4TaNqjvWoADXbJ2Eum5VKy_I5XnvkOLDhHk093FKoZw0kktWs41irFDsTC32ckJnhuRPkGbDmVkSMEsCpiRgODdLAkXy5SyJ0_AaWp_pZWJT-dW_P_8386LyCYNnna4</recordid><startdate>20001101</startdate><enddate>20001101</enddate><creator>Baqui, Munira M. A</creator><creator>Gereben, Balazs</creator><creator>Harney, John W</creator><creator>Larsen, P. Reed</creator><creator>Bianco, Antonio C</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope></search><sort><creationdate>20001101</creationdate><title>Distinct Subcellular Localization of Transiently Expressed Types 1 and 2 Iodothyronine Deiodinases as Determined by Immunofluorescence Confocal Microscopy</title><author>Baqui, Munira M. A ; Gereben, Balazs ; Harney, John W ; Larsen, P. Reed ; Bianco, Antonio C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3102-e689873aaa8a7037f4bda520a7efa0ca327e34f272f6845d08e2af89f3a55b4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino acid sequence</topic><topic>BiP protein</topic><topic>Confocal microscopy</topic><topic>D2 protein</topic><topic>Differential distribution</topic><topic>Endoplasmic reticulum</topic><topic>Epitopes</topic><topic>Flags</topic><topic>Immunofluorescence</topic><topic>Localization</topic><topic>Microscopy</topic><topic>Neuroblastoma</topic><topic>Protein transport</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baqui, Munira M. A</creatorcontrib><creatorcontrib>Gereben, Balazs</creatorcontrib><creatorcontrib>Harney, John W</creatorcontrib><creatorcontrib>Larsen, P. Reed</creatorcontrib><creatorcontrib>Bianco, Antonio C</creatorcontrib><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baqui, Munira M. A</au><au>Gereben, Balazs</au><au>Harney, John W</au><au>Larsen, P. Reed</au><au>Bianco, Antonio C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinct Subcellular Localization of Transiently Expressed Types 1 and 2 Iodothyronine Deiodinases as Determined by Immunofluorescence Confocal Microscopy</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><date>2000-11-01</date><risdate>2000</risdate><volume>141</volume><issue>11</issue><spage>4309</spage><epage>4312</epage><pages>4309-4312</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><abstract>We compared the subcellular localization of FLAG-epitope tagged Types 1
and 2 deiodinases (D1 and D2) transiently expressed in human embryonic
kidney (HEK-293) and mouse neuroblastoma (NB2A) cells. D2 is an
integral membrane protein based on resistance to extraction at pH 11
with the NH2 terminus in the endoplasmic reticulum (ER).
Immunofluorescence confocal microscopy using anti-FLAG and
anti-GRP78/BiP antibodies showed the FLAG-D1 signal was found in the
periphery of the cells and not co-localized with the ER specific marker
GRP78/BiP. On the other hand, FLAG-D2 protein was found in the ER
co-localized with the GRP78/BiP protein. These differential
distribution patterns indicate subcellular sorting of D1 and D2 is
determined by intrinsic protein sequence and can explain the ready
access of D2-generated T3 to the nucleus.</abstract><cop>Washington</cop><pub>Endocrine Society</pub><doi>10.1210/endo.141.11.7872</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Endocrinology (Philadelphia), 2000-11, Vol.141 (11), p.4309-4312 |
| issn | 0013-7227 1945-7170 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Amino acid sequence BiP protein Confocal microscopy D2 protein Differential distribution Endoplasmic reticulum Epitopes Flags Immunofluorescence Localization Microscopy Neuroblastoma Protein transport Proteins |
| title | Distinct Subcellular Localization of Transiently Expressed Types 1 and 2 Iodothyronine Deiodinases as Determined by Immunofluorescence Confocal Microscopy |
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