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Synergism Between FSH and Activin in the Regulation of Proliferating Cell Nuclear Antigen (PCNA) and Cyclin D2 Expression in Rat Granulosa Cells
Follicular development is associated with both proliferation and differentiation of granulosa cells under the control of FSH. We show that regulation of genes involved in cellular proliferation by FSH can be functionally separated from the regulation of genes involved in granulosa cell differentiati...
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| Published in: | Endocrinology (Philadelphia) 2001-10, Vol.142 (10), p.4357-4362 |
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| cites | cdi_FETCH-LOGICAL-c3768-2442abd4519f7594f83cab73c2fac8423bbb9745ed0b5956dd4b0fd74a276e313 |
| container_end_page | 4362 |
| container_issue | 10 |
| container_start_page | 4357 |
| container_title | Endocrinology (Philadelphia) |
| container_volume | 142 |
| creator | El-Hefnawy, Talal Zeleznik, Anthony J |
| description | Follicular development is associated with both proliferation and
differentiation of granulosa cells under the control of FSH. We show
that regulation of genes involved in cellular proliferation by FSH can
be functionally separated from the regulation of genes involved in
granulosa cell differentiation by synergistic actions of activin and T.
Incubation of undifferentiated rat granulosa cells with FSH, forskolin,
activin-A, or T alone did not influence either the expression of the
proliferation-associated genes cyclin D2 and proliferating cell nuclear
antigen or the differentiation-associated genes P450 aromatase, LH
receptor, P450 cholesterol side-chain cleavage enzyme, and
3β-hydroxysteroid dehydrogenase. However, when granulosa cells were
stimulated with either FSH or forskolin in the presence of activin-A,
significant increases (P < 0.05) were observed for
cyclin D2 and proliferating cell nuclear antigen at both the mRNA and
protein levels as well as mRNAs for P450 aromatase, LH receptor, P450
cholesterol side-chain cleavage enzyme and 3β-hydroxysteroid
dehydrogenase. Although T synergized with FSH to increase the
expression of mRNAs for P450 aromatase, LH receptor, P450 cholesterol
side-chain cleavage enzyme, and 3β-hydroxysteroid dehydrogenase,
it did not interact with FSH to increase the expression of mRNAs for
cyclin D2 and proliferating cell nuclear antigen. The differences in
the actions of activin and T could provide a cellular
mechanism by which FSH-regulated granulosa cell proliferation
could be functionally separated from FSH-regulated granulosa cell
differentiation. |
| doi_str_mv | 10.1210/endo.142.10.8438 |
| format | article |
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differentiation of granulosa cells under the control of FSH. We show
that regulation of genes involved in cellular proliferation by FSH can
be functionally separated from the regulation of genes involved in
granulosa cell differentiation by synergistic actions of activin and T.
Incubation of undifferentiated rat granulosa cells with FSH, forskolin,
activin-A, or T alone did not influence either the expression of the
proliferation-associated genes cyclin D2 and proliferating cell nuclear
antigen or the differentiation-associated genes P450 aromatase, LH
receptor, P450 cholesterol side-chain cleavage enzyme, and
3β-hydroxysteroid dehydrogenase. However, when granulosa cells were
stimulated with either FSH or forskolin in the presence of activin-A,
significant increases (P < 0.05) were observed for
cyclin D2 and proliferating cell nuclear antigen at both the mRNA and
protein levels as well as mRNAs for P450 aromatase, LH receptor, P450
cholesterol side-chain cleavage enzyme and 3β-hydroxysteroid
dehydrogenase. Although T synergized with FSH to increase the
expression of mRNAs for P450 aromatase, LH receptor, P450 cholesterol
side-chain cleavage enzyme, and 3β-hydroxysteroid dehydrogenase,
it did not interact with FSH to increase the expression of mRNAs for
cyclin D2 and proliferating cell nuclear antigen. The differences in
the actions of activin and T could provide a cellular
mechanism by which FSH-regulated granulosa cell proliferation
could be functionally separated from FSH-regulated granulosa cell
differentiation.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/endo.142.10.8438</identifier><language>eng</language><publisher>Washington: Endocrine Society</publisher><subject>Activin ; Antigens ; Aromatase ; Cell differentiation ; Cell proliferation ; Cholesterol ; Cleavage ; Cyclin D2 ; Dehydrogenase ; Dehydrogenases ; Differentiation (biology) ; Enzymes ; Follicle-stimulating hormone ; Forskolin ; Gene regulation ; Genes ; Granulosa cells ; Hydroxysteroids ; mRNA ; Proliferating cell nuclear antigen ; Receptors ; Synergism</subject><ispartof>Endocrinology (Philadelphia), 2001-10, Vol.142 (10), p.4357-4362</ispartof><rights>Copyright © 2001 by The Endocrine Society 2001</rights><rights>Copyright © 2001 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3768-2442abd4519f7594f83cab73c2fac8423bbb9745ed0b5956dd4b0fd74a276e313</citedby><cites>FETCH-LOGICAL-c3768-2442abd4519f7594f83cab73c2fac8423bbb9745ed0b5956dd4b0fd74a276e313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>El-Hefnawy, Talal</creatorcontrib><creatorcontrib>Zeleznik, Anthony J</creatorcontrib><title>Synergism Between FSH and Activin in the Regulation of Proliferating Cell Nuclear Antigen (PCNA) and Cyclin D2 Expression in Rat Granulosa Cells</title><title>Endocrinology (Philadelphia)</title><description>Follicular development is associated with both proliferation and
differentiation of granulosa cells under the control of FSH. We show
that regulation of genes involved in cellular proliferation by FSH can
be functionally separated from the regulation of genes involved in
granulosa cell differentiation by synergistic actions of activin and T.
Incubation of undifferentiated rat granulosa cells with FSH, forskolin,
activin-A, or T alone did not influence either the expression of the
proliferation-associated genes cyclin D2 and proliferating cell nuclear
antigen or the differentiation-associated genes P450 aromatase, LH
receptor, P450 cholesterol side-chain cleavage enzyme, and
3β-hydroxysteroid dehydrogenase. However, when granulosa cells were
stimulated with either FSH or forskolin in the presence of activin-A,
significant increases (P < 0.05) were observed for
cyclin D2 and proliferating cell nuclear antigen at both the mRNA and
protein levels as well as mRNAs for P450 aromatase, LH receptor, P450
cholesterol side-chain cleavage enzyme and 3β-hydroxysteroid
dehydrogenase. Although T synergized with FSH to increase the
expression of mRNAs for P450 aromatase, LH receptor, P450 cholesterol
side-chain cleavage enzyme, and 3β-hydroxysteroid dehydrogenase,
it did not interact with FSH to increase the expression of mRNAs for
cyclin D2 and proliferating cell nuclear antigen. The differences in
the actions of activin and T could provide a cellular
mechanism by which FSH-regulated granulosa cell proliferation
could be functionally separated from FSH-regulated granulosa cell
differentiation.</description><subject>Activin</subject><subject>Antigens</subject><subject>Aromatase</subject><subject>Cell differentiation</subject><subject>Cell proliferation</subject><subject>Cholesterol</subject><subject>Cleavage</subject><subject>Cyclin D2</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>Differentiation (biology)</subject><subject>Enzymes</subject><subject>Follicle-stimulating hormone</subject><subject>Forskolin</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Granulosa cells</subject><subject>Hydroxysteroids</subject><subject>mRNA</subject><subject>Proliferating cell nuclear antigen</subject><subject>Receptors</subject><subject>Synergism</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqNkF1LwzAYhYMoOD_uvQx4o0hnvrq0l7NuKoiKH9clTd_Oji6ZSavuX_iTTTfBK0EIhJz3nOcNB6EjSoaUUXIOprRDKtgwCIngyRYa0FTEkaSSbKMBIZRHkjG5i_a8n4enEIIP0NfTyoCb1X6BL6D9ADB4-nSNlSnxWLf1e21wOO0r4EeYdY1qa2uwrfCDs01dgQuCmeEMmgbfdboB5fDYtPUscE4esrvx6RqVrXQTMJcMTz6XDrzvKUF4VC2-csp0jfVqTfEHaKdSjYfDn3sfvUwnz9l1dHt_dZONbyPN5SiJmBBMFaWIaVrJOBVVwrUqJNesUjoRjBdFkUoRQ0mKOI1HZSkKUpVSKCZHwCnfR8cb7tLZtw58m89t50xYmYcpiSkdERlcZOPSznrvoMqXrl4ot8opyfve8773PPTeC33vIXK2idhu-R-33Lj7iXa1gXU_v5_5M_kNo6CWbQ</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>El-Hefnawy, Talal</creator><creator>Zeleznik, Anthony J</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope></search><sort><creationdate>20011001</creationdate><title>Synergism Between FSH and Activin in the Regulation of Proliferating Cell Nuclear Antigen (PCNA) and Cyclin D2 Expression in Rat Granulosa Cells</title><author>El-Hefnawy, Talal ; Zeleznik, Anthony J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3768-2442abd4519f7594f83cab73c2fac8423bbb9745ed0b5956dd4b0fd74a276e313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Activin</topic><topic>Antigens</topic><topic>Aromatase</topic><topic>Cell differentiation</topic><topic>Cell proliferation</topic><topic>Cholesterol</topic><topic>Cleavage</topic><topic>Cyclin D2</topic><topic>Dehydrogenase</topic><topic>Dehydrogenases</topic><topic>Differentiation (biology)</topic><topic>Enzymes</topic><topic>Follicle-stimulating hormone</topic><topic>Forskolin</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Granulosa cells</topic><topic>Hydroxysteroids</topic><topic>mRNA</topic><topic>Proliferating cell nuclear antigen</topic><topic>Receptors</topic><topic>Synergism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Hefnawy, Talal</creatorcontrib><creatorcontrib>Zeleznik, Anthony J</creatorcontrib><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Hefnawy, Talal</au><au>Zeleznik, Anthony J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergism Between FSH and Activin in the Regulation of Proliferating Cell Nuclear Antigen (PCNA) and Cyclin D2 Expression in Rat Granulosa Cells</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><date>2001-10-01</date><risdate>2001</risdate><volume>142</volume><issue>10</issue><spage>4357</spage><epage>4362</epage><pages>4357-4362</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><abstract>Follicular development is associated with both proliferation and
differentiation of granulosa cells under the control of FSH. We show
that regulation of genes involved in cellular proliferation by FSH can
be functionally separated from the regulation of genes involved in
granulosa cell differentiation by synergistic actions of activin and T.
Incubation of undifferentiated rat granulosa cells with FSH, forskolin,
activin-A, or T alone did not influence either the expression of the
proliferation-associated genes cyclin D2 and proliferating cell nuclear
antigen or the differentiation-associated genes P450 aromatase, LH
receptor, P450 cholesterol side-chain cleavage enzyme, and
3β-hydroxysteroid dehydrogenase. However, when granulosa cells were
stimulated with either FSH or forskolin in the presence of activin-A,
significant increases (P < 0.05) were observed for
cyclin D2 and proliferating cell nuclear antigen at both the mRNA and
protein levels as well as mRNAs for P450 aromatase, LH receptor, P450
cholesterol side-chain cleavage enzyme and 3β-hydroxysteroid
dehydrogenase. Although T synergized with FSH to increase the
expression of mRNAs for P450 aromatase, LH receptor, P450 cholesterol
side-chain cleavage enzyme, and 3β-hydroxysteroid dehydrogenase,
it did not interact with FSH to increase the expression of mRNAs for
cyclin D2 and proliferating cell nuclear antigen. The differences in
the actions of activin and T could provide a cellular
mechanism by which FSH-regulated granulosa cell proliferation
could be functionally separated from FSH-regulated granulosa cell
differentiation.</abstract><cop>Washington</cop><pub>Endocrine Society</pub><doi>10.1210/endo.142.10.8438</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0013-7227 |
| ispartof | Endocrinology (Philadelphia), 2001-10, Vol.142 (10), p.4357-4362 |
| issn | 0013-7227 1945-7170 |
| language | eng |
| recordid | cdi_proquest_journals_3130511607 |
| source | Oxford Journals Online |
| subjects | Activin Antigens Aromatase Cell differentiation Cell proliferation Cholesterol Cleavage Cyclin D2 Dehydrogenase Dehydrogenases Differentiation (biology) Enzymes Follicle-stimulating hormone Forskolin Gene regulation Genes Granulosa cells Hydroxysteroids mRNA Proliferating cell nuclear antigen Receptors Synergism |
| title | Synergism Between FSH and Activin in the Regulation of Proliferating Cell Nuclear Antigen (PCNA) and Cyclin D2 Expression in Rat Granulosa Cells |
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