Changes in Rubisco Localization and the Activities of C4 Enzymes in Kochia prostrata Under Elevated CO2 and Temperatures

The content and localization of Rubisco large subunit (RbcL), as well as the activities of NADP-MDH and NADP-ME enzymes involved in the C 4 carbon-concentrating mechanism (CCM) were studied under elevated (800 ppm, eCO 2 ) CO 2 concentration at optimal (25°C) and elevated (32°C, eT) temperatures in...

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Published in:Russian journal of plant physiology 2024-12, Vol.71 (6), Article 188
Main Authors: Shuyskaya, E. V., Khalilova, L. A., Prokofieva, M. Yu, Rakhmankulova, Z. F.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Calvin cycle
Carbon dioxide
Carbon dioxide concentration
Enzymes
Kochia prostrata
Life Sciences
Localization
Mesophyll
NADP
Photosynthesis
Plant cells
Plant Physiology
Plant Sciences
Research Papers
Ribulose-bisphosphate carboxylase
Sheaths
Starch
Starch grains
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Summary:The content and localization of Rubisco large subunit (RbcL), as well as the activities of NADP-MDH and NADP-ME enzymes involved in the C 4 carbon-concentrating mechanism (CCM) were studied under elevated (800 ppm, eCO 2 ) CO 2 concentration at optimal (25°C) and elevated (32°C, eT) temperatures in the leaves of C 4 -NADP species Kochia prostrata . In control plants Rubisco was revealed not only in the bundle sheath cells (BS), typical of C 4 plants but also in the mesophyll cells (M), indicating a C 4 -like type of photosynthesis. In addition, the presence of starch grains in the BS and the adjacent layer of mesophyll cells (M(I)) suggests the Calvin cycle activity. eT and/or eCO 2 markedly reduced the number of starch grains in these cells, probably as a result of decreased activity of C 4 CCM (NADP-MDH and NADP-ME activities). NADP-MDH activity was sensitive to temperature, while that of NADP-ME decreased under eT or eCO 2 but most conspicuously when they acted together. Under eCO 2 , regardless of temperature, the appearance of starch grains was observed in the cells of the second mesophyll layer (M(II)), which also indicated Calvin cycle activity. Thus, K . prostrata exhibits the signs of C 4 -like photosynthesis and, possibly, an active C 3 cycle in the M(II) cells under eCO 2 alone or combined with eT.
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443724606724