Effects of Crotalaria spectabilis extract in Meloidogyne javanica and Heterodera glycines soybean nematodes

Nematodes are a common soybean threat, and the search for new solutions for controlling them has been the focus of many studies worldwide. Crotalaria spp. are known sources of pyrrolizidine alkaloids with nematicidal activities. In this study, the nematicidal and nematostatic effects of C. spectabil...

Full description

Saved in:
Bibliographic Details
Published in:European journal of plant pathology 2024-12, Vol.170 (4), p.985-996
Main Authors: Scupinari, Tamires, Mannochio-Russo, Helena, Marcheafave, Gustavo Galo, da Silva Bolzani, Vanderlan, de Oliveira Nunes, Estela, Dias, Waldir Pereira, Hoffmann-Campo, Clara Beatriz, Zeraik, Maria Luiza
Format: Article
Language:English
Subjects:
Agriculture
Alkaloids
Bioactive compounds
Bioassays
Biomedical and Life Sciences
Design factors
Design of experiments
Ecology
Experimental design
Heterodera glycines
High performance liquid chromatography
Life Sciences
Liquid chromatography
Meloidogyne javanica
Monocrotaline
Mortality
Nematodes
Plant extracts
Plant Pathology
Plant Sciences
Pyrrolizidine alkaloids
Senecionine
Soybeans
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nematodes are a common soybean threat, and the search for new solutions for controlling them has been the focus of many studies worldwide. Crotalaria spp. are known sources of pyrrolizidine alkaloids with nematicidal activities. In this study, the nematicidal and nematostatic effects of C. spectabilis extracts were evaluated in vitro over two different nematode species ( Heterodera glycines and Meloidogyne javanica ). A full factorial experimental design was employed to understand the effects and interactions of input factors (plant organ, extraction solvent, and nematode species) on the output response (nematode mortality). Ethanolic and alkaline extracts of leaves and seeds were tested at concentrations of 5, 10 and 20 mg L -1 in bioassays with 48 hours of incubation. The C. spectabilis seed’s alkaline extract assessed for H. glycines resulted in higher mortality rates (close to 96%) compared to the other experiments. Therefore, this combination was also evaluated in vivo in a soybean plantation, resulting in a decrease of about 90% in the nematode multiplication factor compared to the control group. Ultra-High Performance Liquid Chromatography coupled to a tandem High-Resolution Mass Spectrometer (UHPLC-HRMS/MS) analyses revealed pyrrolizidine alkaloids in the extract, such as senecionine N -oxide, besides monocrotaline and its derivatives. The results obtained in this study allow new possibilities to handle nematode infections. The direct application of C. spectabilis extracts or their isolated bioactive compounds on soil can be a promising approach.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-024-02821-4