Potential Benefits of Physalis minima Stem Water Extract on Bone Health in Ovariectomized Rats: Osteoblast, Osteoclast, and Malondialdehyde Modulation

Around 50% of women over 50 with menopause show osteoporosis symptoms, increasing fracture risk. Estrogen deficiency from menopause affects bone remodeling. Groundcherry (Physalis minima L.) has phytoestrogens. We studied groundcherry stem water extract (GSWE) on femur osteoblasts, osteoclasts, and...

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Published in:Research journal of pharmacy and technology 2024-09, Vol.17 (9), p.4373-4380
Main Authors: Harsachatri, Deasy Ovi, Nugrahenny, Dian, Soeharto, Setyawati, Permatasari, Nur
Format: Article
Language:English
Subjects:
Abdomen
Acids
Alcohol
Antibiotics
Antioxidants
Birth control
Bones
Endocrine therapy
Estrogens
Fractures
Menopause
Oophorectomy
Osteoporosis
Oxidative stress
Womens health
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Nugrahenny, Dian
Soeharto, Setyawati
Permatasari, Nur
description Around 50% of women over 50 with menopause show osteoporosis symptoms, increasing fracture risk. Estrogen deficiency from menopause affects bone remodeling. Groundcherry (Physalis minima L.) has phytoestrogens. We studied groundcherry stem water extract (GSWE) on femur osteoblasts, osteoclasts, and malondialdehyde (MDA) in ovariectomized rats. Rats were grouped: sham-operated, 5-week ovariectomized, 9-week ovariectomized, and three GSWE-treated ovariectomized groups (500, 1500, and 2500mg/kgBW for four weeks). Hematoxylin-eosin staining quantified osteoblast and osteoclast numbers (1000x magnification) in right femur bones. Left femur bones were powdered for MDA analysis via thiobarbituric acid reactive substances (TBARS) test. For osteoblasts, 5-week (21.25±0.957) and 9-week ovariectomized rats (22.75±1.708) had reduced (p
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Estrogen deficiency from menopause affects bone remodeling. Groundcherry (Physalis minima L.) has phytoestrogens. We studied groundcherry stem water extract (GSWE) on femur osteoblasts, osteoclasts, and malondialdehyde (MDA) in ovariectomized rats. Rats were grouped: sham-operated, 5-week ovariectomized, 9-week ovariectomized, and three GSWE-treated ovariectomized groups (500, 1500, and 2500mg/kgBW for four weeks). Hematoxylin-eosin staining quantified osteoblast and osteoclast numbers (1000x magnification) in right femur bones. Left femur bones were powdered for MDA analysis via thiobarbituric acid reactive substances (TBARS) test. For osteoblasts, 5-week (21.25±0.957) and 9-week ovariectomized rats (22.75±1.708) had reduced (p<0.01) osteoblast numbers compared to sham (33.75±2.754). GSWE-treated ovariectomized rats at 500 (46.00±1.414) and 1500 mg/kg BW (56.25±6.701) showed increased (p<0.0001) osteoblast numbers, but the 2500 mg/kg BW group had fewer osteoblast numbers (25.50±1.291), similar to the level seen in untreated ovariectomized rats (p>0.05). For osteoclasts, 5-week (25.00±0.816) and 9-week ovariectomized rats (29.50±1.732) had more (p<0.0001) osteoclast numbers compared to sham (17.75±1.893). Conversely, GSWEtreated ovariectomized rats showed fewer (p<0.01) osteoclast numbers at all doses than untreated ovariectomized rats. A significant negative correlation between GSWE doses and osteoclast numbers was observed (r=-0.595; p=0.015). MDA levels were higher (p<0.0001) in 9-week ovariectomized rats (0.115±0.020) than in sham (0.017±0.014). GSWE-treated ovariectomized rats had lower (p<0.0001) MDA levels at all doses than untreated ovariectomized rats. A negative correlation between GSWE doses and MDA levels was evident (r=-0.611; p=0.012). MDA levels correlated negatively with osteoblast numbers (r=-0.577; p=0.019) and positively with osteoclasts (r=0.790; p<0.0001). In conclusion, GSWE could potentially enhance bone health by elevating osteoblast numbers, reducing osteoclast numbers, and lowering MDA levels. These findings suggest its potential as a natural intervention for addressing menopause-related bone issues. 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Estrogen deficiency from menopause affects bone remodeling. Groundcherry (Physalis minima L.) has phytoestrogens. We studied groundcherry stem water extract (GSWE) on femur osteoblasts, osteoclasts, and malondialdehyde (MDA) in ovariectomized rats. Rats were grouped: sham-operated, 5-week ovariectomized, 9-week ovariectomized, and three GSWE-treated ovariectomized groups (500, 1500, and 2500mg/kgBW for four weeks). Hematoxylin-eosin staining quantified osteoblast and osteoclast numbers (1000x magnification) in right femur bones. Left femur bones were powdered for MDA analysis via thiobarbituric acid reactive substances (TBARS) test. For osteoblasts, 5-week (21.25±0.957) and 9-week ovariectomized rats (22.75±1.708) had reduced (p<0.01) osteoblast numbers compared to sham (33.75±2.754). GSWE-treated ovariectomized rats at 500 (46.00±1.414) and 1500 mg/kg BW (56.25±6.701) showed increased (p<0.0001) osteoblast numbers, but the 2500 mg/kg BW group had fewer osteoblast numbers (25.50±1.291), similar to the level seen in untreated ovariectomized rats (p>0.05). For osteoclasts, 5-week (25.00±0.816) and 9-week ovariectomized rats (29.50±1.732) had more (p<0.0001) osteoclast numbers compared to sham (17.75±1.893). Conversely, GSWEtreated ovariectomized rats showed fewer (p<0.01) osteoclast numbers at all doses than untreated ovariectomized rats. A significant negative correlation between GSWE doses and osteoclast numbers was observed (r=-0.595; p=0.015). MDA levels were higher (p<0.0001) in 9-week ovariectomized rats (0.115±0.020) than in sham (0.017±0.014). GSWE-treated ovariectomized rats had lower (p<0.0001) MDA levels at all doses than untreated ovariectomized rats. A negative correlation between GSWE doses and MDA levels was evident (r=-0.611; p=0.012). MDA levels correlated negatively with osteoblast numbers (r=-0.577; p=0.019) and positively with osteoclasts (r=0.790; p<0.0001). In conclusion, GSWE could potentially enhance bone health by elevating osteoblast numbers, reducing osteoclast numbers, and lowering MDA levels. These findings suggest its potential as a natural intervention for addressing menopause-related bone issues. 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Estrogen deficiency from menopause affects bone remodeling. Groundcherry (Physalis minima L.) has phytoestrogens. We studied groundcherry stem water extract (GSWE) on femur osteoblasts, osteoclasts, and malondialdehyde (MDA) in ovariectomized rats. Rats were grouped: sham-operated, 5-week ovariectomized, 9-week ovariectomized, and three GSWE-treated ovariectomized groups (500, 1500, and 2500mg/kgBW for four weeks). Hematoxylin-eosin staining quantified osteoblast and osteoclast numbers (1000x magnification) in right femur bones. Left femur bones were powdered for MDA analysis via thiobarbituric acid reactive substances (TBARS) test. For osteoblasts, 5-week (21.25±0.957) and 9-week ovariectomized rats (22.75±1.708) had reduced (p<0.01) osteoblast numbers compared to sham (33.75±2.754). GSWE-treated ovariectomized rats at 500 (46.00±1.414) and 1500 mg/kg BW (56.25±6.701) showed increased (p<0.0001) osteoblast numbers, but the 2500 mg/kg BW group had fewer osteoblast numbers (25.50±1.291), similar to the level seen in untreated ovariectomized rats (p>0.05). For osteoclasts, 5-week (25.00±0.816) and 9-week ovariectomized rats (29.50±1.732) had more (p<0.0001) osteoclast numbers compared to sham (17.75±1.893). Conversely, GSWEtreated ovariectomized rats showed fewer (p<0.01) osteoclast numbers at all doses than untreated ovariectomized rats. A significant negative correlation between GSWE doses and osteoclast numbers was observed (r=-0.595; p=0.015). MDA levels were higher (p<0.0001) in 9-week ovariectomized rats (0.115±0.020) than in sham (0.017±0.014). GSWE-treated ovariectomized rats had lower (p<0.0001) MDA levels at all doses than untreated ovariectomized rats. A negative correlation between GSWE doses and MDA levels was evident (r=-0.611; p=0.012). MDA levels correlated negatively with osteoblast numbers (r=-0.577; p=0.019) and positively with osteoclasts (r=0.790; p<0.0001). In conclusion, GSWE could potentially enhance bone health by elevating osteoblast numbers, reducing osteoclast numbers, and lowering MDA levels. These findings suggest its potential as a natural intervention for addressing menopause-related bone issues. Further research can explore its applications.]]></abstract><cop>Raipur</cop><pub>A&amp;V Publications</pub><doi>10.52711/0974-360X.2024.00676</doi></addata></record>
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subjects Abdomen
Acids
Alcohol
Antibiotics
Antioxidants
Birth control
Bones
Endocrine therapy
Estrogens
Fractures
Menopause
Oophorectomy
Osteoporosis
Oxidative stress
Womens health
title Potential Benefits of Physalis minima Stem Water Extract on Bone Health in Ovariectomized Rats: Osteoblast, Osteoclast, and Malondialdehyde Modulation
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