Mining and utilization of mushroom ESTs for microsatellites

In the present study we report on the exploitation of expressed sequence tags (ESTs); (1) to investigate whether microsatellite densities are significantly differed among Pleurotus ostreatus , Lentinula edodes and Agaricus bisporus ESTs, (2) between development stages of mycelia and fruiting bodies...

Full description

Saved in:
Bibliographic Details
Published in:Conservation genetics 2010-06, Vol.11 (3), p.1123-1126
Main Authors: Polat, Ersin, Ince, Ayse Gul, Karaca, Mehmet, Naci Onus, A.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation biology
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Genetic markers
Life Sciences
Mushrooms
Plant Genetics and Genomics
Plant populations
Population genetics
Technical Note
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study we report on the exploitation of expressed sequence tags (ESTs); (1) to investigate whether microsatellite densities are significantly differed among Pleurotus ostreatus , Lentinula edodes and Agaricus bisporus ESTs, (2) between development stages of mycelia and fruiting bodies and (3) to identify microsatellite primer pairs that could be used in mushroom genetic studies. Analyses of ESTs indicated that three mushroom species and tissues showed statistically significant microsatellite densities. A total of 23 EST-microsatellite primer pairs were developed and tested on two species of mushrooms. The use of these microsatellite primer pairs could be used in genetic studies of mushroom species.
ISSN:1566-0621
1572-9737
DOI:10.1007/s10592-009-9896-y