Composition, Vigor, and Proteome of Mature Soybean Seeds Developed under High Temperature

The effects of high temperature treatment on soybean [Glycine max (L.) Merr.] seed composition, vigor, and proteome were investigated using mature dry seeds harvested from plants grown in environment-controlled chambers. High day/night temperatures (37/30°C) from stages R5 through R8 altered ratios...

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Bibliographic Details
Published in:Crop science 2009-05, Vol.49 (3), p.1010-1022
Main Authors: Ren, C, Bilyeu, K.D, Beuselinck, P.R
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
air temperature
Biological and medical sciences
cell respiration
Economic plant physiology
Environmental conditions
fatty acid composition
Fatty acids
filling period
Fundamental and applied biological sciences. Psychology
gene expression
Glycine max
heat stress
High temperature
Membranes
phytic acid
plant proteins
protein content
protein synthesis
Proteinase inhibitors
Proteins
proteome
proteomics
seed germination
Seeds
Soybeans
Studies
Sucrose
sugars
Temperature effects
vigor
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Summary:The effects of high temperature treatment on soybean [Glycine max (L.) Merr.] seed composition, vigor, and proteome were investigated using mature dry seeds harvested from plants grown in environment-controlled chambers. High day/night temperatures (37/30°C) from stages R5 through R8 altered ratios of individual fatty acids to total fatty acid compared to the control (27/18°C). Concentration of sugars decreased, but total protein and phytic acid concentration were unchanged. High temperature resulted in a greater proportion of abnormal seeds, but normal-appearing seed exhibited reduced germination and vigor. Proteomic analysis detected 20 protein identities whose accumulations were changed by the high temperature. Fourteen spots were identified as seven subunits of seed storage proteins. The remaining six proteins were identified as those responding to abiotic stresses or having a function in respiration: (i) sucrose binding protein, (ii) Class III acidic endochitinase, (iii) heat shock protein (HSP22), (iv) late embryo abundant protein, (v) Bowman-Birk proteinase inhibitor, and (vi) formate dehydrogenase. High temperature during seed development changed soybean seed composition and decreased seed vigor, but also changed seed protein expression profiles.
ISSN:0011-183X
1435-0653
DOI:10.2135/cropsci2008.05.0247