Evaluating the biological activity of rolB-transgenic Nicotiana tabacum plants using phytochemical and pharmacological approaches

The present study explored the impact of rolB gene transformation on biological activities of Nicotiana tabacum. PCR, and RT-qPCR were used to validate the integration of rolB gene and its expression. HPLC analysis was used for phytochemical profiling, and various in-vitro and in-vivo assays were us...

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Published in:Plant biosystems 2024-11, Vol.158 (6), p.1428-1442
Main Authors: Anwar, Afshan, Haneef, Shabana, Ismail, Hammad, Bhatti, Muhammad Zeeshan, Ullah, Nazif, Murtaza, Iram, Mirza, Bushra
Format: Article
Language:English
Subjects:
Acetylcholinesterase
Agrobacterium rhizogenes
Analgesics
Biological activity
Damage detection
Data analysis
DNA damage
Flavonoids
Free radicals
Genetic transformation
In vitro methods and tests
Liquid chromatography
Nicotiana tabacum
Pharmacology
Phenolic compounds
Phenols
Phytochemicals
Plant extracts
polyphenols
RolB gene
Scavenging
Transgenic plants
Variance analysis
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Summary:The present study explored the impact of rolB gene transformation on biological activities of Nicotiana tabacum. PCR, and RT-qPCR were used to validate the integration of rolB gene and its expression. HPLC analysis was used for phytochemical profiling, and various in-vitro and in-vivo assays were used to assess pharmacological activities of wild-type and transgenic extracts using ANOVA and Tukey's multiple comparison method for data analysis. Our findings indicated increased total phenolic content (24.4 ± 0.2 mg GAE/g DW of extract) and total flavonoid content (58.8 ± 1.1 mg QE/g DW of extract) in transgenic plants. In case of antioxidant activities, an increase in reducing power (51.82 ± 0.4 AAE mg/g), and radical scavenging (IC 50 188 ± 2.7 µg/mL) was observed. Similarly, transgenic lines exhibited DNA protection against damage caused by free radicals. Moreover, we detected significant antileishmanial potential (IC 50 350.7 ± 8.3 µg/mL), butyrylcholinesterase (IC 50 268.6 ± 4.7 µg/mL), and acetylcholinesterase inhibitions (IC 50 421.6 ± 3.7 µg/mL) in transgenic plants. Similarly, compared with the non-transgenic plants, the rolB-transgenic plants showed increased analgesic (71.4%), anti-inflammatory activities (77.8%), and 35.6% decrease in depression compared to non-transgenic plants. The improved pharmacological activities in transgenic plants have been linked to the level of phytochemicals that increase the therapeutic and curative properties of N. tabacum plants.
ISSN:1126-3504
1724-5575
DOI:10.1080/11263504.2024.2421234