Micropropagation and genetic transformation of Byblis liniflora

• Published in: In vitro cellular & developmental biology. Plant 2024-10, Vol.60 (5), p.634-645
• Main Authors: Coronado-Martín, Alberto, Martin-Vásquez, Constanza, Jáquez, Marybel, Bahaji, Abdellatif, Atarés, Alejandro
• Format: Article
• Language: English
• Subjects: Acclimatization; Acetic acid; Biomedical and Life Sciences; Byblis; Cell Biology; Chemical composition; Chemical compounds; Chromosomes; Culture techniques; Developmental Biology; Explants; Fluorescence; Genetic analysis; Genetic transformation; Glycosides; Green fluorescent protein; Indoleacetic acid; Life Sciences; Micropropagation; Pharmaceuticals; Pharmacology; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Sciences; Propagation; Regeneration; Seeds; Sucrose; Transgenes; Transgenic plants; Zeatin
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-024-10448-7

Byblis , a small genus of carnivorous plants predominantly found in Australia, is characterized by its passive trapping mechanism and unique floral features. The chemical composition of Byblis , including identified phenylethanoid glycosides, particularly acteoside, highlights its pharmacological potential with various biological activities.  In vitro  culture techniques have been established for propagation, with micropropagation protocols developed for different Byblis species. However, information on genetic transformation, vital for trait modification and enhanced pharmacological interest, remains limited. This study focuses on optimizing micropropagation, adventitious regeneration, and genetic transformation methods for  Byblis liniflora . Adventitious regeneration rates were highest in medium with reduced Murashige and Skoog salts (MS/10) and sucrose (3 gL −1 ) concentrations. Zeatin supplementation (1 mgL −1 ) further improved regeneration rates and bud development with 100% of regenerated root explants and 8.8 shoots per explant. Liquid MB3 medium supplemented with indole-3-acetic acid (IAA) 5 mgL −1 facilitated efficient rooting and acclimatization. The establishment of an efficient Rhizobium -mediated genetic transformation method yielded transgenic plants expressing green fluorescent protein (GFP). Molecular analysis confirmed transgene integration, marking the first successful genetic transformation in the Byblis genus. These advancements pave the way for exploring gene function and enhancing pharmacological properties, thereby broadening our understanding and utilization of carnivorous plants like Byblis .