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Evaluation of C/N ratios (glucose/nitrate) for heterotrophic cultivation of a Brazilian strain of Tetradesmus obliquus: An assessment of heterotrophic, mixotrophic, and photoautotrophic conditions
The growth of microalgae under different trophic modes is widely considered in the literature. However, the cultivation of some microalgal strains under heterotrophic conditions requires further investigation. Thus, the present study investigated the growth potential of a Brazilian strain of Tetrade...
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| Published in: | Journal of applied phycology 2024-09, Vol.36 (6), p.3187-3197 |
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| container_title | Journal of applied phycology |
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| creator | Vieira, Renata Augusto Owatari, Marco Shizuo Lopes, Rafael Garcia Derner, Roberto Bianchini |
| description | The growth of microalgae under different trophic modes is widely considered in the literature. However, the cultivation of some microalgal strains under heterotrophic conditions requires further investigation. Thus, the present study investigated the growth potential of a Brazilian strain of
Tetradesmus obliquus
(Chlorophyceae, Scenedesmaceae) in terms of heterotrophic metabolism. For this purpose, cultures were generated using different concentrations of glucose (26, 13, and 6.5 g L
–1
) and sodium nitrate (0.6 g, 0.3, and 0.15 g L
–1
) in culture media. The growth parameters analysed were the maximum biomass achieved and volumetric productivity. The combination of 26 g L
–1
glucose with 0.6 g L
–1
sodium nitrate resulted in the greatest increase in dry weight (9.63 ± 0.15 g L
–1
). Then,
T. obliquus
cultures under heterotrophic conditions were compared with those under photoautotrophic and mixotrophic conditions. The maximum production in heterotrophic cultivation was similar to what had been obtained, while mixotrophic cultivation presented the best result, 14.77 ± 0.06 g L
–1
, and photoautotrophic cultivation obtained a maximum biomass of 7.90 ± 0.17 g L
–1
. The productivity values achieved were 0.52 ± 0.04 g L
–1
day
–1
, 0.98 ± 0.07 g L
–1
day
–1
, and 1.24 ± 0.07 g L
–1
day
–1
for photoautotrophic, heterotrophic and mixotrophic cultures, respectively. The results indicated that the Brazilian microalga
T. obliquus
has the potential to be successfully cultivated heterotrophically.
Graphical Abstract |
| doi_str_mv | 10.1007/s10811-024-03348-1 |
| format | article |
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Tetradesmus obliquus
(Chlorophyceae, Scenedesmaceae) in terms of heterotrophic metabolism. For this purpose, cultures were generated using different concentrations of glucose (26, 13, and 6.5 g L
–1
) and sodium nitrate (0.6 g, 0.3, and 0.15 g L
–1
) in culture media. The growth parameters analysed were the maximum biomass achieved and volumetric productivity. The combination of 26 g L
–1
glucose with 0.6 g L
–1
sodium nitrate resulted in the greatest increase in dry weight (9.63 ± 0.15 g L
–1
). Then,
T. obliquus
cultures under heterotrophic conditions were compared with those under photoautotrophic and mixotrophic conditions. The maximum production in heterotrophic cultivation was similar to what had been obtained, while mixotrophic cultivation presented the best result, 14.77 ± 0.06 g L
–1
, and photoautotrophic cultivation obtained a maximum biomass of 7.90 ± 0.17 g L
–1
. The productivity values achieved were 0.52 ± 0.04 g L
–1
day
–1
, 0.98 ± 0.07 g L
–1
day
–1
, and 1.24 ± 0.07 g L
–1
day
–1
for photoautotrophic, heterotrophic and mixotrophic cultures, respectively. The results indicated that the Brazilian microalga
T. obliquus
has the potential to be successfully cultivated heterotrophically.
Graphical Abstract</description><identifier>ISSN: 0921-8971</identifier><identifier>EISSN: 1573-5176</identifier><identifier>DOI: 10.1007/s10811-024-03348-1</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Algae ; Aquatic microorganisms ; Biomass ; Biomedical and Life Sciences ; Carbon-nitrogen ratio ; Cultivation ; Culture media ; Cultures ; Dry weight ; Ecology ; Freshwater & Marine Ecology ; Glucose ; Glucose metabolism ; Life Sciences ; Metabolism ; Microalgae ; Nitrates ; Phytoplankton ; Plant Physiology ; Plant Sciences ; Productivity ; Sodium ; Sodium nitrate ; Sodium nitrates ; Tetradesmus obliquus</subject><ispartof>Journal of applied phycology, 2024-09, Vol.36 (6), p.3187-3197</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024 Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>Copyright Springer Nature B.V. Dec 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c200t-2fc3e993e5e4e364d67c0674d89821b96c99baec4112f222ef0a84a065d0aebd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Vieira, Renata Augusto</creatorcontrib><creatorcontrib>Owatari, Marco Shizuo</creatorcontrib><creatorcontrib>Lopes, Rafael Garcia</creatorcontrib><creatorcontrib>Derner, Roberto Bianchini</creatorcontrib><title>Evaluation of C/N ratios (glucose/nitrate) for heterotrophic cultivation of a Brazilian strain of Tetradesmus obliquus: An assessment of heterotrophic, mixotrophic, and photoautotrophic conditions</title><title>Journal of applied phycology</title><addtitle>J Appl Phycol</addtitle><description>The growth of microalgae under different trophic modes is widely considered in the literature. However, the cultivation of some microalgal strains under heterotrophic conditions requires further investigation. Thus, the present study investigated the growth potential of a Brazilian strain of
Tetradesmus obliquus
(Chlorophyceae, Scenedesmaceae) in terms of heterotrophic metabolism. For this purpose, cultures were generated using different concentrations of glucose (26, 13, and 6.5 g L
–1
) and sodium nitrate (0.6 g, 0.3, and 0.15 g L
–1
) in culture media. The growth parameters analysed were the maximum biomass achieved and volumetric productivity. The combination of 26 g L
–1
glucose with 0.6 g L
–1
sodium nitrate resulted in the greatest increase in dry weight (9.63 ± 0.15 g L
–1
). Then,
T. obliquus
cultures under heterotrophic conditions were compared with those under photoautotrophic and mixotrophic conditions. The maximum production in heterotrophic cultivation was similar to what had been obtained, while mixotrophic cultivation presented the best result, 14.77 ± 0.06 g L
–1
, and photoautotrophic cultivation obtained a maximum biomass of 7.90 ± 0.17 g L
–1
. The productivity values achieved were 0.52 ± 0.04 g L
–1
day
–1
, 0.98 ± 0.07 g L
–1
day
–1
, and 1.24 ± 0.07 g L
–1
day
–1
for photoautotrophic, heterotrophic and mixotrophic cultures, respectively. The results indicated that the Brazilian microalga
T. obliquus
has the potential to be successfully cultivated heterotrophically.
Graphical Abstract</description><subject>Algae</subject><subject>Aquatic microorganisms</subject><subject>Biomass</subject><subject>Biomedical and Life Sciences</subject><subject>Carbon-nitrogen ratio</subject><subject>Cultivation</subject><subject>Culture media</subject><subject>Cultures</subject><subject>Dry weight</subject><subject>Ecology</subject><subject>Freshwater & Marine Ecology</subject><subject>Glucose</subject><subject>Glucose metabolism</subject><subject>Life Sciences</subject><subject>Metabolism</subject><subject>Microalgae</subject><subject>Nitrates</subject><subject>Phytoplankton</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Productivity</subject><subject>Sodium</subject><subject>Sodium nitrate</subject><subject>Sodium nitrates</subject><subject>Tetradesmus obliquus</subject><issn>0921-8971</issn><issn>1573-5176</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kc1uEzEUhS0EEqHwAqwssQGJIb62MzNm10blR6pgU9aW47nTuJrYqa9dAc_HgzFpEBEbVvdH5ztncRh7CeIdCNEtCUQP0AipG6GU7ht4xBaw6lSzgq59zBbCSGh608FT9ozoVghheugX7NflvZuqKyFFnka-Xn7h-XARf30zVZ8IlzGU-YVv-Jgy32LBnEpO-23w3NephPu_tOMX2f0MU3CR0wyFh-81zuuAtKvE02YKd7XSe34euSNCoh3GcpD94_yW78L30-HiwPfbVJKr5RSe4hAO0fScPRndRPjizzxj3z5cXq8_NVdfP35en181XgpRGjl6hcYoXKFG1eqh7bxoOz30ppewMa03ZuPQawA5SilxFK7XTrSrQTjcDOqMvTr67nO6q0jF3qaa4xxpFehOGq2MmlXyqPI5EWUc7T6Hncs_LAh7aMse27JzW_ahLQszpI4QzeJ4g_lk_R_qN4w3nX8</recordid><startdate>20240911</startdate><enddate>20240911</enddate><creator>Vieira, Renata Augusto</creator><creator>Owatari, Marco Shizuo</creator><creator>Lopes, Rafael Garcia</creator><creator>Derner, Roberto Bianchini</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20240911</creationdate><title>Evaluation of C/N ratios (glucose/nitrate) for heterotrophic cultivation of a Brazilian strain of Tetradesmus obliquus: An assessment of heterotrophic, mixotrophic, and photoautotrophic conditions</title><author>Vieira, Renata Augusto ; Owatari, Marco Shizuo ; Lopes, Rafael Garcia ; Derner, Roberto Bianchini</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c200t-2fc3e993e5e4e364d67c0674d89821b96c99baec4112f222ef0a84a065d0aebd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Algae</topic><topic>Aquatic microorganisms</topic><topic>Biomass</topic><topic>Biomedical and Life Sciences</topic><topic>Carbon-nitrogen ratio</topic><topic>Cultivation</topic><topic>Culture media</topic><topic>Cultures</topic><topic>Dry weight</topic><topic>Ecology</topic><topic>Freshwater & Marine Ecology</topic><topic>Glucose</topic><topic>Glucose metabolism</topic><topic>Life Sciences</topic><topic>Metabolism</topic><topic>Microalgae</topic><topic>Nitrates</topic><topic>Phytoplankton</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Productivity</topic><topic>Sodium</topic><topic>Sodium nitrate</topic><topic>Sodium nitrates</topic><topic>Tetradesmus obliquus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vieira, Renata Augusto</creatorcontrib><creatorcontrib>Owatari, Marco Shizuo</creatorcontrib><creatorcontrib>Lopes, Rafael Garcia</creatorcontrib><creatorcontrib>Derner, Roberto Bianchini</creatorcontrib><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal of applied phycology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vieira, Renata Augusto</au><au>Owatari, Marco Shizuo</au><au>Lopes, Rafael Garcia</au><au>Derner, Roberto Bianchini</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of C/N ratios (glucose/nitrate) for heterotrophic cultivation of a Brazilian strain of Tetradesmus obliquus: An assessment of heterotrophic, mixotrophic, and photoautotrophic conditions</atitle><jtitle>Journal of applied phycology</jtitle><stitle>J Appl Phycol</stitle><date>2024-09-11</date><risdate>2024</risdate><volume>36</volume><issue>6</issue><spage>3187</spage><epage>3197</epage><pages>3187-3197</pages><issn>0921-8971</issn><eissn>1573-5176</eissn><abstract>The growth of microalgae under different trophic modes is widely considered in the literature. However, the cultivation of some microalgal strains under heterotrophic conditions requires further investigation. Thus, the present study investigated the growth potential of a Brazilian strain of
Tetradesmus obliquus
(Chlorophyceae, Scenedesmaceae) in terms of heterotrophic metabolism. For this purpose, cultures were generated using different concentrations of glucose (26, 13, and 6.5 g L
–1
) and sodium nitrate (0.6 g, 0.3, and 0.15 g L
–1
) in culture media. The growth parameters analysed were the maximum biomass achieved and volumetric productivity. The combination of 26 g L
–1
glucose with 0.6 g L
–1
sodium nitrate resulted in the greatest increase in dry weight (9.63 ± 0.15 g L
–1
). Then,
T. obliquus
cultures under heterotrophic conditions were compared with those under photoautotrophic and mixotrophic conditions. The maximum production in heterotrophic cultivation was similar to what had been obtained, while mixotrophic cultivation presented the best result, 14.77 ± 0.06 g L
–1
, and photoautotrophic cultivation obtained a maximum biomass of 7.90 ± 0.17 g L
–1
. The productivity values achieved were 0.52 ± 0.04 g L
–1
day
–1
, 0.98 ± 0.07 g L
–1
day
–1
, and 1.24 ± 0.07 g L
–1
day
–1
for photoautotrophic, heterotrophic and mixotrophic cultures, respectively. The results indicated that the Brazilian microalga
T. obliquus
has the potential to be successfully cultivated heterotrophically.
Graphical Abstract</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10811-024-03348-1</doi><tpages>11</tpages></addata></record> |
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| subjects | Algae Aquatic microorganisms Biomass Biomedical and Life Sciences Carbon-nitrogen ratio Cultivation Culture media Cultures Dry weight Ecology Freshwater & Marine Ecology Glucose Glucose metabolism Life Sciences Metabolism Microalgae Nitrates Phytoplankton Plant Physiology Plant Sciences Productivity Sodium Sodium nitrate Sodium nitrates Tetradesmus obliquus |
| title | Evaluation of C/N ratios (glucose/nitrate) for heterotrophic cultivation of a Brazilian strain of Tetradesmus obliquus: An assessment of heterotrophic, mixotrophic, and photoautotrophic conditions |
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