Identification of the P24 Gene Family Related to Vesicular Transport in Cyclocarya paliurus and Their Expression Analysis Under Salt Stress

• Published in: Forests 2024-11, Vol.15 (12), p.2081
• Main Authors: Pan, Yuxin, Fei, Chunpeng, Liu, Xin, He, Fayin, Zhu, Jiana, Yu, Hui, Zhao, Fusheng, Yang, Zhengting
• Format: Article
• Language: English
• Subjects: Abiotic stress; Adaptation; Antioxidants; Cell fusion; Chromosomes; Combined treatment; Conserved sequence; Cytoplasm; Environmental conditions; Environmental stress; Gene expression; Genes; Genomes; Herbal medicine; Localization; Medicinal plants; Methyl jasmonate; Osmosis; Osmotic pressure; Oxidative stress; Physiology; Plant growth; Plant layout; Proteins; Research projects; Salinity; Salinity tolerance; Salt; Salts; Signal transduction; Single-nucleotide polymorphism; Sodium; Sodium nitroprusside; Software; Toxicity; Traditional Chinese medicine; Water balance
• ISSN: 1999-4907; 1999-4907
• DOI: 10.3390/f15122081

Vesicle transport is a fundamental mechanism for intracellular substance transfer and signal transduction, involving the formation, transport, fusion with target membranes, and release of vesicle contents within cells. Issues such as disruption of water balance, difficulty in regulating osmotic pressure, and oxidative stress caused by salt stress can lead to disturbances in the secretion system of plants, thus affecting plant growth and development. Cyclocarya paliurus (C. paliurus), widely used in traditional Chinese medicine, has not been previously reported in terms of how the vesicle transport P24 gene regulates its adaptation to salt stress. In this research project, a total of eight CpP24 genes were successfully identified. Upon examination of gene architecture and conserved sequence elements, the CpP24 genes exhibited a variation in exon count, ranging from 4 to 6. Moreover, the CpP24 gene family’s reaction to salt stress and specific stressors including methyl jasmonate (MeJA), sodium hydrosulfide (NaHS), and sodium nitroprusside (SNP) was further explored in our study. Comprehensive analysis of the expression patterns of CpP24 genes under various conditions showed that salt stress induced the expression of these genes, and the combined treatment of salt stress with specific stresses caused changes in their expression. This study lays a theoretical foundation for further probing into the physiological functions of C. paliurus and the underlying mechanisms regarding its response to environmental stress.