The Selection and Validation of Reference Genes for RT-qPCR Analysis of the Predatory Natural Enemy Orius nagaii (Hemiptera: Anthocoridae)

Simple SummaryA predatory natural enemy insect, Orius nagaii, has been discovered in both the northern and southern regions of China and is extensively utilized for greenhouse vegetable pest management. The precise selection of reference genes is essential for RT-qPCR analysis to elucidate the expre...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2024-11, Vol.15 (12), p.936
Main Authors: Wang, Chengxing, Yin, Zhenjuan, Wang, Yu, Liu, Yan, Zhao, Shan, Dai, Xiaoyan, Wang, Ruijuan, Long, Su, Chen, Hao, Li, Zheng, Zhai, Yifan
Format: Article
Language:English
Subjects:
Dehydrogenases
Developmental stages
Eggs
Environmental stress
Gene expression
Genes
Genetic engineering
Insects
Natural enemies
Orius nagaii
Pest control
Proteins
Quantitative analysis
reference gene
RT-qPCR
selection
Stability analysis
Temperature
Transcription factors
validation
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Summary:Simple SummaryA predatory natural enemy insect, Orius nagaii, has been discovered in both the northern and southern regions of China and is extensively utilized for greenhouse vegetable pest management. The precise selection of reference genes is essential for RT-qPCR analysis to elucidate the expression patterns of key genes implicated in the interaction between O. nagaii and its host. This study assessed the expression stability of ten candidate reference genes (CRGs) across various biological conditions and environmental stresses. Our findings revealed that the ideal reference genes were RPS10 and RPL32 at multiple stages of development, while RPS10 and RPS15 proved to be the most suitable combination for various tissues and host diets. The optimal reference gene pairing under temperature-induced stress was EF1-α and RPL6. These outcomes will offer dependable reference genes for subsequent investigations into the gene expression patterns of target genes in O. nagaii and closely related species.
ISSN:2075-4450
DOI:10.3390/insects15120936