Bioassay-Guided Fractionation Networking for Discovery of Biofungicides from Cultivated Salvia canariensis

• Published in: International journal of molecular sciences 2024-12, Vol.25 (24), p.13323
• Main Authors: Hernández-Álvarez, Eduardo, Rodríguez-Sabina, Samuel, González-Delgado, Guacimara, Reyes, Carolina P, Giménez, Cristina, Llaría-López, María Ángeles, Cabrera, Raimundo, Bazzocchi, Isabel L, Jiménez, Ignacio A
• Format: Article
• Language: English
• Subjects: Agricultural production; Alternaria - drug effects; Alternaria - growth & development; Bioassays; Biological Assay - methods; Biological control; Biomass; Biopesticides; Botrytis - drug effects; Botrytis - growth & development; Chromatography; Crop diseases; Ecosystem components; Flavonoids; Food security; Fractionation; Fungi; Fungicides; Fusarium - drug effects; Fusarium - growth & development; Lead compounds; Medicine, Botanic; Medicine, Herbal; Metabolites; Natural products; Pest control; Pesticides; Pests; Phytochemicals; Plant diseases; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Salvia - chemistry; Tetracycline; Tetracyclines
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms252413323

Considering the detrimental impacts of the current pesticides on the biotic components of the biosphere, the development of novel pesticides is vital. Plant-derived biopesticides have emerged as popular alternatives to create a safer and more sustainable agriculture model. This study aims to validate the previous bioguided fractionation of endemic Canary Islands sage, , as a potential source of botanical pesticides using a cultivation process. Accordingly, the bioassay-guided fractionation of the ethanolic extract of the leaves of cultivated on the phytopathogenic fungal mycelia of , , and yielded six known terpenoids. Their abietane diterpenoid-type ( - ) and sesquiterpenoid ( ) structures were established based on spectroscopic and spectrometric analysis. This strategy identified one abietane diterpenoid, salviol ( ), as a potential candidate for the future development of biofungicides with similar potency towards the assayed phytopathogenic fungi to commercial fungicides. Salviol worked in a concentration-dependent manner. Overall, this study reinforces the potential of abietane-type diterpenoids as promising agrochemical lead compounds against infectious diseases caused by phytopathogenic fungi and validates the cultivation of as a potential source of plant-derived biopesticides.