Transcriptome analysis of the effects of Porphyra yezoensis polysaccharide on non-specific immunity of spotted sea bass, Lateolabrax maculatus

• Published in: Aquaculture international 2025, Vol.33 (1), p.103, Article 103
• Main Authors: Lin, Hao, Zhou, Sishun, Ma, Jianrong, Kong, Lumin, Huang, Zhangfan, Liu, Longhui, Zhao, Yanbo, Lin, Yi, Li, Zhongbao
• Format: Article
• Language: English
• Subjects: Aquatic animals; Bass; Biomedical and Life Sciences; Feeds; Freshwater & Marine Ecology; Kidneys; Lateolabrax maculatus; Life Sciences; Lysozyme; Marine fishes; Phosphatase; Polysaccharides; Porphyra yezoensis; Pronephros; Spleen; Transcriptome; Zoology
• ISSN: 0967-6120; 1573-143X
• DOI: 10.1007/s10499-024-01775-y

In order to investigate the effects of dietary Porphyra yezoensis polysaccharide (PP) on the non-specific immunity of spotted sea bass ( Lateolabrax maculatus ), 360 spotted sea bass (10.53 ± 0.02 g) were randomly divided into six groups and fed six different doses of PP (0, 3, 6, 9, 12, and 15 g/kg), respectively. The experiment lasted for 52 days. The results of non-specific immune indexes showed that compared with the control group, the activities of acid phosphatase (ACP) and alkaline phosphatase (AKP) in serum of the 6 and 9 g/kg groups were significantly increased, and the activity of lysozyme (LZM) in each experimental groups was also significantly increased; in the liver, ACP activity in 3 and 6 g/kg groups was significantly increased, and LZM activity in 9 and 12 g/kg groups was significantly increased; in the head kidney, ACP and LZM activities in 9 g/kg group were significantly increased; in the spleen, ACP, AKP, and LZM activities were significantly increased in the 9 g/kg group. The transcriptomic sequencing results of the head kidney showed that PP could improve the immunity of spotted sea bass by regulating the expression of MHC Class I antigen (MHC1), glucocorticoid receptor (NR3C1), small subunit ribosomal protein S3Ae (RPS3A), heme oxygenase 1 (HMOX1), and ferritin heavy chain (FTH1) genes. The results of this study indicate that 9–12 g/kg PP can improve the non-specific immunity of spotted sea bass and provide a theoretical basis for the application of PP in aquatic animal feed.