New Horizons for Temperature Sensing and Bioimaging Using Er3+‐Doped Core@Shell Nanoparticles and Effects of H2O and D2O Solvents on Their Spectroscopic Properties

• Published in: Advanced optical materials 2025-01, Vol.13 (2), p.n/a
• Main Authors: Ryszczyńska, Sylwia, Soler‐Carracedo, Kevin, Ekner‐Grzyb, Anna, Jurga, Natalia, Ćwierzona, Maciej, Piątkowski, Dawid, Grzyb, Tomasz
• Format: Article
• Language: English
• Subjects: Absorption spectra; Banded structure; bioimaging; Biological properties; Blue shift; colloids; Core-shell structure; Emission analysis; Emission spectra; Emission spectroscopy; Erbium; Excitation spectra; Fluorides; lanthanide ions; luminescence; Medical imaging; Medical research; Nanoparticles; nanothermometry; Sodium compounds; Solvents; Temperature dependence; upconversion
• ISSN: 2195-1071; 2195-1071
• DOI: 10.1002/adom.202402053

Lanthanide‐doped nanoparticles (NPs) exhibit temperature‐dependent luminescence, enabling the design of luminescent nanothermometers for industrial and medical applications. This research demonstrates the temperature‐sensing properties of NaYF4:7.5%Er3+@NaYF4 and NaErF4@NaYF4 NPs, which have a hexagonal shape and average size of 17 nm. Their core@shell structure is confirmed using high‐resolution transmission electron microscopy, and they exhibit intense upconversion (UC) emission under 1532 nm excitation in H2O and D2O colloids. The recorded spectra show Er3+ emission bands with varying intensity ratios depending on the Er3+ concentration, chosen solvent, and temperature. The spectroscopic properties of the studied NPs allow for their excitation and observation of emission within biological windows, which makes them useful for bio‐related applications. The emission of prepared NPs is analyzed as a function of temperature from 298 up to 358/363 K in H2O and D2O. The ratios for thermally‐coupled levels  and non‐TCLs and their relative sensitivities are studied. For the high dopant concentration sample in water, the O─H vibrations and blue shift in the absorption spectrum lead to a record relative sensitivity of 2.50% K−1 (at 363 K) for the 2H11/2/4I11/2 ratio. The use of synthesized NPs for bioimaging under 1550 nm excitation is also demonstrated to observe their accumulation in the guts of Daphnia magna. This study presents the synthesis and characterization of Er3+‐doped core@shell NaYF4:7.5%Er3+@NaYF4 and NaErF4@NaYF4 nanoparticles, highlighting their upconversion (UC) properties in H2O and D2O. The work explores the effects of these solvents on spectroscopic behavior, emphasizing applications in temperature sensing and bioimaging. The study also showcases the potential of these nanoparticles for biological imaging under 1550 nm excitation.