Citicoline and coenzyme Q10 (COQUN Combo) reduce inflammation in an experimental glaucoma model

• Published in: Acta ophthalmologica (Oxford, England) England), 2025-01, Vol.103 (S284), p.n/a
• Main Authors: Matamoros, José A., Fernández‐Albarral, José A., Salobrar‐Garcia, Elena, Elvira‐Hurtado, Lorena, López‐Cuenca, Inés, Sanchez‐Puebla, Lidia, Martínez‐López, Miguel A., Rubio‐Casado, Sara, Hoz, Rosa, Gallardo, Meritxell Lopez, Marco, Eva M., Ramirez, Jose Manuel, Salazar, Juan Jose, De La Villa, Pedro, Ramirez, Ana Isabel
• Format: Article
• Language: English
• Subjects: Cell body; Cell differentiation; Citicoline; Coenzyme Q10; Down-regulation; Eye; Gelatin; Glaucoma; Inflammation; Lasers; Microglia; Retina
• ISSN: 1755-375X; 1755-3768
• DOI: 10.1111/aos.17112

Aims/Purpose: To investigate the anti‐inflammatory effects of citicoline and coenzyme Q10 (COQUN Combo) in an experimental model of ocular hypertension (OHT). Methods: Swiss albino mice were used: vehicle control (Vehicle n = 6), COQUN Combo control (COQUN Combo n = 5), laser vehicle (LG+Veh, n = 6), and laser COQUN Combo (LG+COQUN Combo, n = 6). COQUN Combo was administered daily with gelatin 15 days prior to laser induction and 3 days post‐induction in COQUN Combo and LG+COQUN Combo groups. The Vehicle and LG+Veh groups received neutral gelatin. OHT was induced in the left eye by laser photocoagulation of the limbal and episcleral veins, analyzing hypertensive eyes and their contralateral eyes. Retinal whole‐mounts were processed with: 1) Anti Iba‐1 to quantify: Number of Iba‐1+ cells (Iba1‐cn) in outer segments (OS), outer plexiform layer (OPL) and inner plexiform layer (IPL); Retinal area occupied by Iba‐1+ cells (Iba1‐RA) in the nerve fiber layer‐ganglion cell layer (NFL‐GCL); Cell body area (Iba1‐cba) of Iba‐1+ cells in OPL, IPL and NFL‐GCL; and arbor area (Iba1‐aa) of Iba‐1+ cells in OPL and IPL. 2) Anti‐P2RY12 to differentiate activated microglia. Results: No significant differences were observed between the control groups. Both OHT eyes and their contralaterals of LG+Veh, as well as LG+COQUN Combo OHT eyes and their contralaterals, showed significant differences compared to controls in Iba1‐cn, Iba1‐ RA, Iba1‐cba, and Iba1‐aa in all layers analyzed. However, these changes were significantly smaller in LG+COQUN Combo OHT and their contralateral eyes compared to LG+Veh OHT and their contralateral eyes. In the Vehicle, Iba‐1+ cells were P2RY12+. P2RY12 expression was downregulated in both OHT eyes of LG+Veh and LG+ COQUN Combo; however, this downregulation was less pronounced in the OHT eyes of LG+COQUN Combo. In the contralateral eyes, P2RY12 expression remained similar to that of the controls Conclusions: COQUN Combo can attenuate the inflammatory process in glaucoma.