Putative Arabidopsis Transcriptional Adaptor Protein (PROPORZ1) is required to modulate histone acetylation in response to auxin

• Published in: Proceedings of the National Academy of Sciences - PNAS 2010-06, Vol.107 (22), p.10308-10313
• Main Authors: Anzola, Jeanette Moulinier, Sieberer, Tobias, Ortbauer, Martina, Butt, Haroon, Korbei, Barbara, Weinhofer, Isabelle, Müllner, Almuth Elise, Luschnig, Christian
• Format: Article
• Language: English
• Subjects: Acetylation; adaptor proteins; Arabidopsis; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Auxins; Base Sequence; Biological Sciences; Cell cycle; Cell growth; Cell proliferation; Cells; Chromatin; Chromatin remodeling; Circuits; Cyclin-dependent kinase; cyclin-dependent kinase inhibitor; Cyclin-Dependent Kinase Inhibitor Proteins - genetics; Cyclin-Dependent Kinase Inhibitor Proteins - metabolism; Differentiation; DNA Primers - genetics; DNA, Plant - genetics; Effects; enzyme inhibitors; Flowers & plants; Gene expression; gene expression regulation; Gene Expression Regulation, Plant - genetics; genes; Genes, Plant; Genetic loci; Genotype & phenotype; Histones; Histones - chemistry; Histones - metabolism; Hormones; Indoleacetic Acids - metabolism; Indoleacetic Acids - pharmacology; Kip protein; KRP gene; loci; Models, Biological; Molecular Sequence Data; Morphogenesis; Mutation; Plant Growth Regulators - metabolism; Plant Growth Regulators - pharmacology; Plants; Plants, Genetically Modified; PRZ1 gene; Sequence Homology, Nucleic Acid; Stem cells; Transcription; Transcription Factors - genetics; Transcription Factors - metabolism; transcriptional adaptor protein; Translation
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0913918107

Plant development is highly adaptable and controlled by a combination of various regulatory circuits that integrate internal and environmental cues. The phytohormone auxin mediates such growth responses, acting as a dynamic signal in the control of morphogenesis via coordinating the interplay between cell cycle progression and cell differentiation. Mutants in the chromatin-remodeling component PROPORZ1 (PRZ1; also known as AtADA2b) are impaired in auxin effects on morphogenesis, suggestive of an involvement of PRZ1-dependent control of chromatin architecture in the determination of hormone responses. Here we demonstrate that PRZ1 is required for accurate histone acetylation at auxin-controlled loci. Specifically, PRZ1 is involved in the modulation of histone modifications and corresponding adjustments in gene expression of Arabidopsis KIP RELATED PROTEIN (KRP) CDK inhibitor genes in response to auxin. Deregulated KRP expression in KRP silencer lines phenocopies prz1 hyperproliferative growth phenotypes, whereas in a KRP overexpression background some mutant phenotypes are suppressed. Collectively, our findings support a model in which translation of positional signals into developmental cues involves adjustments in chromatin modifications that orchestrate auxin effects on cell proliferation.