Occurrence and characterization of psychrotolerant hydrocarbon-oxidizing bacteria from surface seawater along the Victoria Land coast (Antarctica)

• Published in: Polar biology 2010-07, Vol.33 (7), p.929-943
• Main Authors: Lo Giudice, A, Casella, P, Caruso, C, Mangano, S, Bruni, V, De Domenico, M, Michaud, L
• Format: Article
• Language: English
• Subjects: Aliphatic hydrocarbons; Analysis; Animal and plant ecology; Animal, plant and microbial ecology; Bacteria; Biodegradation; Biological and medical sciences; Biomedical and Life Sciences; Chemical analysis; Ecology; Energy sources; Ethylenediaminetetraacetic acid; Fundamental and applied biological sciences. Psychology; Hydrocarbons; Life Sciences; Low temperature; Microbiology; Oceanography; Original Paper; Particular ecosystems; Phylogenetics; Phylogeny; Plant Sciences; Sea-water; Seawater; Surface water; Synecology; Water analysis; Zoology
• ISSN: 0722-4060; 1432-2056
• DOI: 10.1007/s00300-010-0770-7

A total of 253 hydrocarbon-oxidizing bacterial isolates were achieved from eight Antarctic surface seawater samples enriched on diesel oil at 4°C. Isolates were screened by amplified ribosomal DNA restriction analysis prior to 16S rRNA gene sequencing. Sequences were compared to those in available databases using the Basic Local Alignment Search Tool network service to determine their approximate phylogenetic affiliations. The majority of the isolates were affiliated to the Actinobacteria (75.9%) and the Gamma-Proteobacteria (22.9%). The Alpha- and Beta-Proteobacteria represented 0.8 and 0.4% of total isolates, respectively. The Actinobacteria were predominantly allocated to the genera Arthrobacter, Cryobacterium and Rhodococcus. The Gamma-Proteobacteria were mainly found to be related to the genus Pseudomonas. Conversely, the Alpha- and Beta-Proteobacterial isolates shared the highest degree of sequence identity with unclassified bacteria. Differences in the distribution of the detected phylotypes were observed among the analyzed samples. Isolates representing each phylotype were selected for further characterization, including phenotypic assays and screening for the growth ability in the presence of individual hydrocarburic substrates as the sole supplied carbon and energy source. Isolates possessed different patterns of substrate utilization. Aliphatic hydrocarbons supported the growth of a higher number of isolates than aromatics. Results confirm the ability of our Antarctic marine bacteria to utilize hydrocarbons at low temperature and therefore suggesting that isolates with different substrate specificities can act in nature as a consortium in the utilization of complex hydrocarburic mixtures.