Monitoring of microbial souring in chemically treated, produced-water biofilm systems using molecular techniques

The identification of bacteria in oil production facilities has previously been based on culture techniques. However, cultivation of bacteria from these often-extreme environments can lead to errors in identifying the microbial community members. In this study, molecular techniques including fluores...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2005-04, Vol.32 (4), p.163-170
Main Authors: KJELLERUP, B. V, VEEH, R. H, SUMITHRARATNE, P, THOMSEN, T. R, BUCKINGHAM-MEYER, K, FRØLUND, B, STURMAN, P
Format: Article
Language:English
Subjects:
Bacteria
Biofilms
Biofilms - growth & development
Biological and medical sciences
Bioreactors
Biotechnology
Chemical treatment
Culture Media
Culture techniques
Electrophoresis - methods
Fuel Oils
Fundamental and applied biological sciences. Psychology
In Situ Hybridization, Fluorescence
Industrial Waste
Microbiology
Molecular Sequence Data
Molybdenum - metabolism
Molybdenum - pharmacology
Nitrates
Nitrates - metabolism
Nitrates - pharmacology
Nitrites - metabolism
Nitrites - pharmacology
Petroleum production
Phylogeny
Polymerase Chain Reaction
Sequence Analysis, DNA
Sulfate reduction
Sulfates
Sulfides
Sulfides - metabolism
Sulfur-Reducing Bacteria - classification
Sulfur-Reducing Bacteria - genetics
Sulfur-Reducing Bacteria - growth & development
Sulfur-Reducing Bacteria - isolation & purification
Waste Management
Water Microbiology
Water Pollutants, Chemical
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Summary:The identification of bacteria in oil production facilities has previously been based on culture techniques. However, cultivation of bacteria from these often-extreme environments can lead to errors in identifying the microbial community members. In this study, molecular techniques including fluorescence in situ hybridization, PCR, denaturing gradient gel electrophoresis, and sequencing were used to track changes in bacterial biofilm populations treated with nitrate, nitrite, or nitrate+molybdate as agents for the control of sulfide production. Results indicated that nitrite and nitrate+molybdate reduced sulfide production, while nitrate alone had no effect on sulfide generation. No long-term effect on sulfide production was observed. Initial sulfate-reducing bacterial numbers were not influenced by the chemical treatments, although a significant increase in sulfate-reducing bacteria was observed after termination of the treatments. Molecular analysis showed a diverse bacterial population, but no major shifts in the population due to treatment effects were observed.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-005-0222-5