Scale-up of biopesticide production processes using wastewater sludge as a raw material

Studies were conducted on the production of Bacillus thuringiensis (Bt)-based biopesticides to ascertain the performance of the process in shake flasks, and in two geometrically similar fermentors (15 and 150 l) utilizing wastewater sludge as a raw material. The results showed that it was possible t...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2004-12, Vol.31 (12), p.545-552
Main Authors: YEZZA, A, TYAGI, R. D, VALERO, J. R, SURAMPALLI, R. Y, SMITH, J
Format: Article
Language:English
Subjects:
Animals
Bacillus thuringiensis - growth & development
Bacillus thuringiensis - metabolism
Bacillus thuringiensis - physiology
Biological and medical sciences
Bioreactors
Biotechnology
Colony Count, Microbial
Fermentation
Fundamental and applied biological sciences. Psychology
Industrial Microbiology - methods
Larva - growth & development
Lepidoptera - growth & development
Microbiology
Oxygen transfer
Pest Control, Biological
Pesticides
Raw materials
Reproducibility of Results
Serine Endopeptidases - metabolism
Sewage
Sludge
Spores, Bacterial - physiology
Toxins
Waste Disposal, Fluid
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Summary:Studies were conducted on the production of Bacillus thuringiensis (Bt)-based biopesticides to ascertain the performance of the process in shake flasks, and in two geometrically similar fermentors (15 and 150 l) utilizing wastewater sludge as a raw material. The results showed that it was possible to achieve better oxygen transfer in the larger capacity fermentor. Viable cell counts increased by 38-55% in the bioreactor compared to shake flasks. As for spore counts, an increase of 25% was observed when changing from shake flask to fermentor experiments. Spore counts were unchanged in bench (15 l) and pilot scale (5.3-5.5 e(+08) cfu/ml; 150 l). An improvement of 30% in the entomotoxicity potential was obtained at pilot scale. Protease activity increased by two to four times at bench and pilot scale, respectively, compared to the maximum activity obtained in shake flasks. The maximum protease activity (4.1 IU/ml) was obtained in pilot scale due to better oxygen transfer. The Bt fermentation process using sludge as raw material was successfully scaled up and resulted in high productivity for toxin protein yield and a high protease activity.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-004-0176-z