Some peculiarities of the outer membrane composition of marine Gram-negative bacterium Chryseobacterium indoltheticum CIP 103168T

Some peculiarities of the lipid composition of the outer membrane of the marine Gram-negative bacterium Chryseobacterium indoltheticum CIP 103168T have been studied. It is found that C. indoltheticum cells do not contain phosphatidylglycerol. Lipopolysaccharide could not be extracted by conventional...

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Published in:Biochemistry (Moscow). Supplement series A, Membrane and cell biology Membrane and cell biology, 2007-06, Vol.1 (2), p.114-122
Main Authors: Vorob’eva, E. V., Krasikova, I. N., Dmitrenok, A. S., Dmitrenok, P. S., Drozdov, A. L., Reunov, A. V., Lapshina, L. A., Solov’eva, T. F.
Format: Article
Language:English
Subjects:
Acetic acid
Bacteria
Chloroform
Chryseobacterium
Composition
Glucosamine
Gram-negative bacteria
Lipid A
Lipid composition
Lipids
Lipopolysaccharides
Membrane composition
Membranes
Monosaccharides
Phosphatidylglycerol
Polysaccharides
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Summary:Some peculiarities of the lipid composition of the outer membrane of the marine Gram-negative bacterium Chryseobacterium indoltheticum CIP 103168T have been studied. It is found that C. indoltheticum cells do not contain phosphatidylglycerol. Lipopolysaccharide could not be extracted by conventional methods; therefore, lipid A was isolated by hydrolysis of whole bacterial cells with 10% acetic acid with subsequent treatment with a chloroform: methanol mixture. The isolated lipid A has an unusual monosaccharide structure and constitutes 1-phosphate-D-glucosamine acylated with (R)-3-hydroxy-15-methylhexadecanoic and (R)-3-hydroxy-13-methyltetradecanoic acids at C2 and C3 atoms, respectively. The C. indoltheticum cells are shown to have a capsule; its polysaccharide apparently fulfils the LPS function.
ISSN:1990-7478
1990-7494
DOI:10.1134/S1990747807020031