Lipoxygenase pathway of arachidonic acid metabolism in growth control of tumor cells of different type

The influence of inhibitors of different lipoxygenases (LOX) on the growth of human tumor cells with different profiles of synthesized eicosanoids was studied. The studied LOX inhibitors had virtually no influence on the growth of A549 cells actively synthesizing cyclooxygenase and lipoxygenase meta...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2005-12, Vol.70 (12), p.1396-1403
Main Authors: Kudryavtsev, I A, Gudkova, M V, Pavlova, O M, Oreshkin, A E, Myasishcheva, N V
Format: Article
Language:English
Subjects:
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - pharmacology
Adenocarcinoma - metabolism
Arachidonate Lipoxygenases - metabolism
Arachidonic acid
Arachidonic Acid - antagonists & inhibitors
Arachidonic Acid - metabolism
Carcinoma, Squamous Cell - metabolism
Cell proliferation
Cell Proliferation - drug effects
Flavanones - pharmacology
Humans
Hydroxyeicosatetraenoic Acids - pharmacology
Lung Neoplasms - metabolism
Metabolism
Metabolites
Nitrobenzenes - pharmacology
Salicylamides - pharmacology
Sulfonamides - pharmacology
Tumor Cells, Cultured - metabolism
Tumors
Umbelliferones - pharmacology
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Summary:The influence of inhibitors of different lipoxygenases (LOX) on the growth of human tumor cells with different profiles of synthesized eicosanoids was studied. The studied LOX inhibitors had virtually no influence on the growth of A549 cells actively synthesizing cyclooxygenase and lipoxygenase metabolites of arachidonic acid (AA). The inhibitor of 12-LOX, baicalein, significantly inhibited proliferation in cultures of A431 epidermoid carcinoma cells with a characteristic domination of the major lipoxygenase metabolite of AA, 12-hydroxyeicosatetraenoic acid (12-HETE), in the profile of synthesized eicosanoids and reduced to 70% the incorporation of [3H]thymidine into DNA. Treatment of these cultures with 12-HETE virtually restored the growth potential of the tumor cells. The findings suggest that the lipoxygenase metabolite of AA, 12-HETE, is a growth-limiting factor for tumor cells of definite type.
ISSN:0006-2979
1608-3040
DOI:10.1007/s10541-005-0275-0