Molecular Epidemiology of Extended-Spectrum [beta]-Lactamases from Klebsiella pneumoniae Strains Isolated in Zagreb, Croatia

In order to assess the molecular epidemiology of 40 previously identified extended-spectrum β-lactamase (ESBL)-producing clinical isolates of Klebsiella pneumoniae, gene sequencing was performed. While the previous examination of these isolates revealed one TEM producer, the sequencing procedure per...

Full description

Saved in:
Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2001-07, Vol.20 (7), p.505
Main Authors: Bedenic, B, Randegger, C, Stobberingh, E, Hächler, H
Format: Article
Language:English
Subjects:
Epidemiology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to assess the molecular epidemiology of 40 previously identified extended-spectrum β-lactamase (ESBL)-producing clinical isolates of Klebsiella pneumoniae, gene sequencing was performed. While the previous examination of these isolates revealed one TEM producer, the sequencing procedure performed in this study identified 13 additional TEM producers, and all of the sequenced genes reflected production of nonESBL TEM-1. All 38 suspected SHV producers were confirmed to be carriers of bla^sub SHV-ESBL^ genes using the PCR/NheI test and sequencing. Among them, types SHV-2, SHV-5, and SHV-12 were found in 20, 10, and 7 isolates, respectively, and SHV-2a was identified in 1. SHV-5 and SHV-12 conferred higher resistance to ceftazidime and cefepime, while SHV-2 and SHV-2a raised the minimum inhibitory concentrations of cefotaxime and cefpirome. Fourth-generation cephalosporins were found to be more active against the isolates than third-generation cephalosporins.[PUBLICATION ABSTRACT]
ISSN:0934-9723
1435-4373
DOI:10.1007/PL00011293