A two-site immunometric assay for the detection of the expression level of [alpha]2(C2)-adrenergic receptor produced in Escherichia coli

Human α^sub 2^(C2)-adrenergic receptor was expressed in Escherichia coli as a fusion protein with Bacillus circulans var. alcalophilus cyclomaltodextrin glucanotransferase. For the determination of the expression level (0.6 mg of solubilized fusion protein l^sup -1^ of E. coli culture), a two-site i...

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Bibliographic Details
Published in:Biotechnology letters 2000-12, Vol.22 (24), p.1963
Main Authors: Frang, Heini, Hellman, Jukka, Goldman, Adrian, Scheinin, Mika, Glumoff, Tuomo
Format: Article
Language:English
Subjects:
Bacteria
E coli
Proteins
Online Access:Get full text
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Summary:Human α^sub 2^(C2)-adrenergic receptor was expressed in Escherichia coli as a fusion protein with Bacillus circulans var. alcalophilus cyclomaltodextrin glucanotransferase. For the determination of the expression level (0.6 mg of solubilized fusion protein l^sup -1^ of E. coli culture), a two-site immunometric assay based on two monoclonal antibodies with different epitopes was developed.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1026766330471