Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-[gamma] but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli

Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) dri...

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Bibliographic Details
Published in:Biotechnology letters 2007-09, Vol.29 (9), p.1369
Main Authors: Medina-rivero, E, Balderas-hernández, V E, Ordoñez-acevedo, L G, Paz-maldonado, L M, T, Barba-De la Rosa, A P, De León-Rodríguez, A
Format: Article
Language:English
Subjects:
E coli
Penicillin
Translocation
Online Access:Get full text
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Summary:Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1% of hINF-γ was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-007-9395-5