Accumulation of Cd2+ and Pb2+ in the suspension cultures of Agave amaniensis and Costus speciosus and the determination of the culture's growth and phytosteroid content

Cell suspension cultures of Agave amaniensis and Costus speciosus were grown in media containing Cd^sup 2 +^ up to 25 and 20 mg l^sup -1^, respectively, and Pb^sup 2+^ up to 40 mg l^sup -1^. The cultures hyper-accumulated Cd^sup 2+^ up to 900 and 530 μg g^sup -1^ and Pb^sup 2+^ up to 1390 and 1170 μ...

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Bibliographic Details
Published in:Biotechnology letters 2002-05, Vol.24 (9), p.687-690
Main Authors: KARTOSENTONO, Sugijanto, SURYAWATI, Sinar, INDRAYANTO, Gunawan, NOOR CHOLIES ZAINI
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomass
Biotechnology
Cell culture
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Miscellaneous
Plant cells and fungal cells
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Summary:Cell suspension cultures of Agave amaniensis and Costus speciosus were grown in media containing Cd^sup 2 +^ up to 25 and 20 mg l^sup -1^, respectively, and Pb^sup 2+^ up to 40 mg l^sup -1^. The cultures hyper-accumulated Cd^sup 2+^ up to 900 and 530 μg g^sup -1^ and Pb^sup 2+^ up to 1390 and 1170 μg g^sup -1^ dry wt. in their respective biomasses. Increasing Pb^sup 2+^ up to 30 mg l^sup -1^ increased the biomass production and total sitosterol content of Costus speciosus by up to 1.7- and 1.3-fold, respectively.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1015225931409