Lighting E. coli cells as biological sensors for Cd2

Whole cells of E. coli expressing a chimeric cadmium-binding peptide fused to green fluorescent protein (CdBP-GFP) were prepared and applied for the determination of cadmium. Construction of the structural gene was performed by inserting two synthetic oligonucleotides coding for four repeats of a Cd...

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Bibliographic Details
Published in:Biotechnology letters 2001-08, Vol.23 (16), p.1285-1291
Main Authors: PRACHAYASITTIKUL, Virapong, NA AYUDHYA, Chartchalerm Isarankura, BULOW, Leif
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Cadmium
E coli
Environment and pollution
Fundamental and applied biological sciences. Psychology
Heavy metals
Industrial applications and implications. Economical aspects
Metal concentrations
Miscellaneous
Proteins
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Summary:Whole cells of E. coli expressing a chimeric cadmium-binding peptide fused to green fluorescent protein (CdBP-GFP) were prepared and applied for the determination of cadmium. Construction of the structural gene was performed by inserting two synthetic oligonucleotides coding for four repeats of a Cd-binding peptide (His-Ser-Gln-Lys-Val-Phe) into the 5'-end of the GFPuv gene. Similarly, a hexahistidine-green fluorescent protein (his^sub 6^GFPuv) was prepared and used as a reference in the determinations of heavy metals. The lowest concentrations of Cd, which activated the fluorescence, were 0.5 μM, 50 μM, and 0.5 mM for cells carrying CdBP^sub 4^GFP, his^sub 6^GFP and native GFP, respectively.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1010561120720