Improved production of biosurfactant by a Pseudomonas aeruginosa mutant using vegetable oil refinery wastes

Biosurfactant production by Pseudomonas aeruginosa EBN-8 mutant was studied in shake flasks on separate wastes from canola, soybean and corn oil refineries. Of the substrates tested, canola oil refinery waste (COD=20 g l(-1)) supplemented with sodium nitrate (at COD/N=20) showed the best microbial g...

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Bibliographic Details
Published in:Biodegradation (Dordrecht) 2007-02, Vol.18 (1), p.115-121
Main Authors: ZULFIQAR ALI RAZA, REHMAN, Asma, MUHAMMAD SALEEM KHAN, KHALID, Zafar M
Format: Article
Language:English
Subjects:
Biodegradation of pollutants
Biodegradation, Environmental
Biological and medical sciences
Biomass
Biotechnology
Canola Oil
Environment and pollution
Fatty Acids, Monounsaturated - metabolism
Fundamental and applied biological sciences. Psychology
Glycolipids - metabolism
Industrial applications and implications. Economical aspects
Industrial Waste - analysis
Mutation
Nitrates - metabolism
Oil refineries
Petroleum refineries
Plant Oils - metabolism
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - metabolism
Refineries
Refinery wastes
Soybeans
Surface Tension
Surface-Active Agents - metabolism
Vegetable oils
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Summary:Biosurfactant production by Pseudomonas aeruginosa EBN-8 mutant was studied in shake flasks on separate wastes from canola, soybean and corn oil refineries. Of the substrates tested, canola oil refinery waste (COD=20 g l(-1)) supplemented with sodium nitrate (at COD/N=20) showed the best microbial growth (4.50 g l(-1)) and rhamnolipid production (8.50 g l(-1)), at 10 d of incubation with the specific growth rate of 0.316 h(-1) and specific product yield of 0.597 g g(-1) h. Its cell-free supernatant showed the critical micelle dilution (CMD) of 150 and surface tension (ST) of 28.5 mN m(-1).
ISSN:0923-9820
1572-9729
DOI:10.1007/s10532-006-9047-9