Thiomorpholine and morpholine oxidation by a cytochrome P450 in Mycobacterium aurum MO1. Evidence of the intermediates by in situ 1H NMR

Spectrophotometric assays of Mycobacterium aurum MO1 cells extracts gave evidence of a soluble cytochrome P450, involved in the degradative pathway of morpholine, a waste product from the chemical industry. In order to get further information, the kinetics of the biodegradation of the sulfur analogu...

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Bibliographic Details
Published in:Biodegradation (Dordrecht) 1998, Vol.9 (6), p.433-442
Main Authors: COMBOURIEU, B, POUPIN, P, BESSE, P, SANCELME, M, VESCHAMBRE, H, TRUFFAUT, N, DELORT, A.-M
Format: Article
Language:English
Subjects:
Biodegradation
Biodegradation, Environmental
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Biotransformation
Chemical industry
Cytochrome
Cytochrome P-450 Enzyme Inhibitors
Cytochrome P-450 Enzyme System - metabolism
Enzyme Activation - drug effects
Fundamental and applied biological sciences. Psychology
Magnetic Resonance Spectroscopy - methods
Metyrapone - pharmacology
Mission oriented research
Morpholines - metabolism
Mycobacterium - metabolism
NMR
Nuclear magnetic resonance
Oxidation
Oxidation-Reduction
Physiology and metabolism
Spectrophotometry
Sulfur
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Summary:Spectrophotometric assays of Mycobacterium aurum MO1 cells extracts gave evidence of a soluble cytochrome P450, involved in the degradative pathway of morpholine, a waste product from the chemical industry. In order to get further information, the kinetics of the biodegradation of the sulfur analogue thiomorpholine was monitored by using in situ nuclear magnetic resonance (NMR). This technique allowed the identification of two intermediates: the sulfoxide of thiomorpholine resulting from S-oxidation and thiodiglycolic acid owing to ring cleavage. The S-oxidation (S-->SO) represents one of the well-known reactions catalyzed by cytochromes P450. The inhibitory effect of metyrapone, a cytochrome P450 inhibitor, on the thiomorpholine and morpholine degradative abilities of M. aurum MO1 confirmed the involvement of a cytochrome P450. These results and the decrease of the rate of formation of the first intermediate during the morpholine degradation, 2-(2-aminoethoxy) acetate, proved the key role of the cytochrome P450 in the early events of the biodegradation, i.e., in the C-N bond cleavage.
ISSN:0923-9820
1572-9729
DOI:10.1023/A:1008321610465